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Microbio 3
Fichas del conjunto (61)
- 1CFU is a unit that describesBacterial living cell density
- 2A plate with ___ colonies can be recorded as TFTC,Fewer than 30
- 3Plate with ___ can be counted as TNTCMore than 300
- 4What unit is used for density of viral growth?PFU
- 5You dilute a bacteria culture by a factor of 10^4 and spread 200ul on a plate. The next day you count 150 colonies on the plate. What was the original cell density?7.5 x 10^6 CFU/ml
- 6How can a 1000-fold dilution be written?1/1000, 1X10^-3, 10 X 10 ^ -4
- 7Why is serial dilution performed?To attain a countable number of colonies
- 8What did we use to sterilize the hockey stick spreaders in this lab?Ethenol and bunsen burner
- 9When should a student remove glowvestouching door handle to the room
- 10T/F we did a bacteria spread on 2 plates for each dilution to obtain reliable numbers?T
- 11T/F on tuesday, we serialy diulted our bacteria culture 10-fold five times?False
- 12What is bacteria transformation?Bacteria gets genetic information from enviornement
- 13Plasmids are sometimes beneficial to bacteria becauseplasmids may contain antibiotic resistant genes
- 14What is an operon?Structural and functional genetic units of prokaryotes
- 15Green flourescent protei nused as indicator for gene transcription
- 16PlasmidUsed as vector for GFP gene to E.coli
- 17Regulatory molecule for arabinose promotor. Allows RNA to bindAntibiotic resistance gene
- 18Enzyme that hydrolyzes certain antibiotics. differentiates cells.Antibiotic resistant gene
- 19T/F following transformation, the plasmid becomes intergrated into bacteria chromosomes?False
- 20T/F we used plasmid pGLO as a vector to carry GFP protein into bacteriaFalse
- 21A complete arabinose operon consists ofpromotor and 3 structural genes
- 22In an emergency room at the lawrence hospital, which of the following would likely be the biggest drawback to identifying bacteria species using API-20E prodecure we used in class?Long test incubation tome/wait for results because it takes 24 hours
- 23After 24 hours of the API20 test there is no growth. What can you assume about fermentation of nitrate reduction of the test? Is it a member of enterovacteriaceae?Negative for nitrate fermentation and not enterobactericae
- 24Why is agar used?Solidifying agent
- 25Which of the following contained or "displayed" epitopes that recongized in agglutination reaction?The antigen
- 26What are antibodies primarily composed of?Protein
- 27Which of the following are composed of double stranded DNA?pGLO plasmids
- 28What was the green flurocent protein (GFP) composed of?Amino acids
- 29What is the pGLO plasmid composed of?Nucleotides
- 30After E.Coli sucessfully transformed using the pGlo plasmid, where is the gene for GFPInside E.coli but not in bacterial genome
- 31In a typical pGlo transformation procedure, appoximatley what percent of bacteria undergo calcium and heat shock actually end up transformed?<1%
- 32Calcium and heat shock treatment caused ___ in our transformation expirimentpores to form, allowing uptake of DNA
- 33Which type of colonies would most likely to produce GFFP?pGlo-transformed E.coli cultured in presence of amipicillin + Arbinose
- 34Why were the vials ( or transoformed cells) incubated for 10 min in LB nrothe rather than transferring their contentes directly to plates?Allow ampicillin-resistence protein to be produced
- 35What can be fermented?polysacchardies
- 36Which is true about selective media?-aids in isolation
-produced by adding bacteriostatic agent
-produced exculding number of nuitrent sorces from media
-colonies appear isolated could have unseen contaminants - 37Suppose a student testing an unknown culture performs the DNAse test according to the protocol; after 48 hours, the DNAse media has not clarifie d. Which of the following conclusions would be most proper / justified?No DNAse activity observed
- 38The locations where chemically induced DNA replications error occur are?Randomly distributed
- 39How would you expect chemical TOXICITY to affect growth of Ames - strain Salmonella typhimurium ?Prevent growth
- 40How would you expect chemical MUTAGENICITY to affect growth of Ames - strain Salmonella typhimurium ?Induce mutations, reduce auxotrophs
- 41. Which list is ordered from smallest to largest (in ter m s of physical size)?gene, operon, plasmid, porkaryotic, chromosome, eukaryotic chromosome
- 42What is the fundamental, underlying reason why species of bacteria vary with respect to their abilities to utilize different types of carbohydrates?DNA- Fermentation processes are catalyzed by genetically-encoded enzymes
- 43Which of the following factors could be rate - limiting with respect to the rate of bacterial population growth?spatial constrants/compeition, depletion of substrates, starting materials, accumilation of bacteria metabolities, and length
- 44At temperatures ABOVE the cardinal temperature of a bacteria l species , the rate of cell division _____________; at temperatures BELOW that cardinal temperature, the rate of cell division ____________Decresase and decrease
- 45Concentration (cell density) of bacterial in suspension may be estimated using a spectrophotometer. Increasing turbidity is the same as ____________optical density; both indicate _______________ cell densityincreasing, and decreasing
- 46—It is mutagenic
- 47—Has most concentration of chemical testing
- 48In a typical Ames test procedure , approximately what percent of mutations result in rescue of auxotrophic mutants?Very few - less than 1%
- 49"Which plate(s) do you need to examine to determine if your test substance is mutagenic?"Minimal media plate
- 50"Why can't your test substance [the potential mutagen] contain protein?Bacteria could obtain histidine from test substance
- 51The tests we use in this class for differentiation of species during the mixed double unknown project wereQualitative
- 52Were the blue nitrile gloves you used in lab sterile ?No
- 53Aggluination is an interaction between?Antigen and antibody
- 54What is contained in the various regents added to strep sapp during aggulationantibody
- 55Which of the following is a good negative control for grape juice fermentation?Grape juice
- 56What is a possible byproduct of fermentation?Lactic acid and co2
- 57what is the range of acceptable colonies for a standard plate count?30-300
- 58What is the end zone of inhibition called?minimum inhibitory concentration
- 59Which plate would you expect to see GFP (glowing) colonies?LB + ampicillin + Arbinose
- 60Which antibiotic is most effective against antimicrobial agent?10 micrograms of antibiotic and 20 mm zone of inhibition
- 61t/f bacteria resistance to specific antibiotics change over time?True
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- Fichas sobre Microbio 3, creado por brittbartak el 05/05/2014.
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- brittbartak
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