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2.1.3 Measuring objects seen with a light microscope
Descripción
AS level (2.1- Cell Structure) Biology Mapa Mental sobre 2.1.3 Measuring objects seen with a light microscope, creado por Aleena Sheraz el 07/03/2017.
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biology
2.1- cell structure
as level
Mapa Mental por
Aleena Sheraz
, actualizado hace más de 1 año
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Creado por
Aleena Sheraz
hace alrededor de 7 años
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Resumen del Recurso
2.1.3 Measuring objects seen with a light microscope
Using graticules
Microscope eyepiece= fitted with graticule
Graticule= transparent with a small ruler etched on
The scale is arbitrary - represents diff. lengths at diff. magnifications
As a specimen is viewed the eyepiece graticule scale is superimposed and the dimensions of specimen can be measured in epu
Eyepiece scale has to be calibrated for each different objective lens by using a stage graticule
Stage graticule= precise measuring divide with a small scale to calibrate value of eyepiece divisions at different magnifications
Using a stage graticule to calibrate the eyepiece graticule
A stage graticule, microscopic ruler, is placed on the microscope stage
Ruler is 1mm long with 100 divisions= each division is 0.01mm or 10um
1) Insert eyepiece graticule in X10 eyepiece of microscope
2) Place a stage graticule on microscope stage, bring to focus, use low power X4 objective. T. magnification= X40
3) Align eyepiece and stage graticule. Check value of 1 eyepiece division
4) In the example, stage graticule (1000um) corresponds to 40 eyepiece divisions
5) Each eyepiece division= 1000/40 = 25um
6) Now use X10 objective lens (T. magnification is X100) and focus on stage graticule
7) Align both graticules
8) From the example, 100 eyepiece divisions correspond with 1000um
9) So one eyepiece division= 1000/100= 10um
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