Test
Bio 110 Final: Chapter 20
Preguntas del test (10)
1Vectors used to clone genes were derived originally from
- Aproteins
- Bplasmids
- Cviruses
- Dall of the above
- Eb and c only
2Restriction enzymes used in most cloning experiments
- Acut CNA into pieces for gene cloning
- Bare naturally produced by bacteria cells to prevent viral infection
- Cproduce sticky ends on DNA fragments
- DAll of the above are correct
- Eonly a and c are correct
3DNA ligase is needed in a cloning experiment
- Ato promote hydrogen bonding between sticky ends
- Bto catalyze the covalent attachment of the backbone of DNA strands
- Cto digest the chromosomal DNA into small pieces
- DOnly a and b are correct
- Ea, b, and c are correct
4Let's suppose you followed protocols described in figures 20.2 and 20.3. Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector with an inserted fragment of DNA?
- APick a bacterial colony and restreak on plates containing X-Gal to confirm that the cells really form white colonies.
- BPick a bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then preform gel electrophoresis with the DNA.
- CPick a bacterial colony and test it to see it X-Gal is functional within the bacterial cells.
- DPick a bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.
- EBoth c and d should be conducted.
5Why is Taq polymerase used in PCR rather than other DNA polymerases?
- ATaq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
- BTaq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
- CTaq polymerase is easier to isolate than other DNA polymerases.
- DTaq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
- EAll of the above are correct.
6Let's suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements do you agree with the most?
- ADo PCR to clone the gene because it is much faster.
- BDo PCR to clone the gene because it is very specific and gives a high yield.
- CYou can't do PCR because you can't make forward and reverse primers.
- DDo cloning by insertion into a vector because it will give you a higher yield.
- EDo cloning by insertion into a vector because it is easier than PCR.
7The method of determining the base sequence of DNA is termed
- APCR
- Bgene cloning
- CDNA fingerprinting
- DDNA sequencing
- Egene mapping
8With regard to dideoxy sequencing, which of the following statements is false?
- AThe dideoxy nucleotides are fluorescently labeled.
- BThe dideoxy nucleotides cannot be incorporated into a growing DNA strand.
- CWhen incorporated into a DNA strand, the dideoxynucleotides prevent further growth of the strand.
- DThe dideoxy sequencing method is used to determine the base sequence of DNA.
- EThe dideoxy sequencing method requires the use of primers.
9Organisms that carry genes that were introduced using molecular techniques are called
- Atransgenics
- Brecombinant DNA
- Cmutants
- Dgenetically modified organisms
- Eboth a and d
10DNA fingerprinting is used
- Ato precisely identify an organism, such as the identification of specific strains of bacteria
- Bas a forensics tool to provide evidence in a criminal case
- Cto determine the genetic relationship between individuals
- Dto determine the identity of an individual
- Efor all of the above.
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