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Quiz

Bio 110 Final: Chapter 20

Usuário eliminado 10 perguntas Atualizado em 13 dez 2017

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Perguntas do quiz (10)

  1. 1Vectors used to clone genes were derived originally from
    1. Aproteins
    2. Bplasmids
    3. Cviruses
    4. Dall of the above
    5. Eb and c only
  2. 2Restriction enzymes used in most cloning experiments
    1. Acut CNA into pieces for gene cloning
    2. Bare naturally produced by bacteria cells to prevent viral infection
    3. Cproduce sticky ends on DNA fragments
    4. DAll of the above are correct
    5. Eonly a and c are correct
  3. 3DNA ligase is needed in a cloning experiment
    1. Ato promote hydrogen bonding between sticky ends
    2. Bto catalyze the covalent attachment of the backbone of DNA strands
    3. Cto digest the chromosomal DNA into small pieces
    4. DOnly a and b are correct
    5. Ea, b, and c are correct
  4. 4Let's suppose you followed protocols described in figures 20.2 and 20.3. Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector with an inserted fragment of DNA?
    1. APick a bacterial colony and restreak on plates containing X-Gal to confirm that the cells really form white colonies.
    2. BPick a bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then preform gel electrophoresis with the DNA.
    3. CPick a bacterial colony and test it to see it X-Gal is functional within the bacterial cells.
    4. DPick a bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.
    5. EBoth c and d should be conducted.
  5. 5Why is Taq polymerase used in PCR rather than other DNA polymerases?
    1. ATaq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
    2. BTaq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
    3. CTaq polymerase is easier to isolate than other DNA polymerases.
    4. DTaq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
    5. EAll of the above are correct.
  6. 6Let's suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements do you agree with the most?
    1. ADo PCR to clone the gene because it is much faster.
    2. BDo PCR to clone the gene because it is very specific and gives a high yield.
    3. CYou can't do PCR because you can't make forward and reverse primers.
    4. DDo cloning by insertion into a vector because it will give you a higher yield.
    5. EDo cloning by insertion into a vector because it is easier than PCR.
  7. 7The method of determining the base sequence of DNA is termed
    1. APCR
    2. Bgene cloning
    3. CDNA fingerprinting
    4. DDNA sequencing
    5. Egene mapping
  8. 8With regard to dideoxy sequencing, which of the following statements is false?
    1. AThe dideoxy nucleotides are fluorescently labeled.
    2. BThe dideoxy nucleotides cannot be incorporated into a growing DNA strand.
    3. CWhen incorporated into a DNA strand, the dideoxynucleotides prevent further growth of the strand.
    4. DThe dideoxy sequencing method is used to determine the base sequence of DNA.
    5. EThe dideoxy sequencing method requires the use of primers.
  9. 9Organisms that carry genes that were introduced using molecular techniques are called
    1. Atransgenics
    2. Brecombinant DNA
    3. Cmutants
    4. Dgenetically modified organisms
    5. Eboth a and d
  10. 10DNA fingerprinting is used
    1. Ato precisely identify an organism, such as the identification of specific strains of bacteria
    2. Bas a forensics tool to provide evidence in a criminal case
    3. Cto determine the genetic relationship between individuals
    4. Dto determine the identity of an individual
    5. Efor all of the above.

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O usuário eliminou as informações de disciplina Quiz sobre Bio 110 Final: Chapter 20, criado por Usuário eliminado em 13-12-2017.
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Usuário eliminado
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English
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Criado em 13 dez 2017 · atualizado em 13 dez 2017

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