13148521
Description
| Question | Answer |
| What is interpupillary distance? | The distance between the two eyepieces of a binocular microscope. These should be adjusted by the individual using the microscope until the views merge and they see a single image. Different people have different interpupillary distances. |
| Which two factors comprise the total magnification of a microscope? | Eyepiece magnification (usually x10) multiplied by the objective magnification (the one closest to the slide being viewed). |
| What is the condenser of a microscope and how is it adjusted? |
Image:
Scope1 (image/jpeg)
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| What is the rationale for performing Kohler illumination? | Doing this allows you to get the optimum amount of light through the specimen you are viewing. |
| How is Kohler illumination performed? | Focus a microscope on a specimen at the lowest objective magnification (x4). Then close the field iris until the illuminated area is about the size of a 20p piece, smaller than the field of view with a dark area surrounding it. Now adjust the condenser control until the edges of the 20p piece are in sharp focus. Place the illuminated area in the centre of the field of view using the right and the left condenser centering screws. Open up the field iris until the edges of the 20p piece disappear and the illuminated area fills the entire field of view. Finally, adjust the condenser iris until the best contrast and resolution for the specimen is created. The condenser iris will need slight readjustment with each change of objective lens. |
| How can bacteria be fixed onto a slide? |
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