Unit 2: Genetic Control Of Metabolism

Question	Answer
What Is Mutagensis?	Mutagensis is the process of inducing mutantions in an organisms genome through exposure to uv light or mutagenic chemicals & radiation
What Technology Is Used To Transfer DNA From One Species To Another?	Recombinant DNA Technology
How Does It Work?	Recombinant DNA Technology involves the use of recombinant plasmids and artificial chromosomes as vectors
What Molecules Are Used For Foreign DNA Insertion?	Plasmids are used for short fragments of DNA Artifical chromosomes are preferable for larger fragments of foreign DNA
What Is A Restriction Endonucleases?	An Enzyme
What Does It Do?	Restriction endonuclease cut open plasmids & specific genes out of chromosomes leaving sticky ends
How Are Complementary Sticky Ends Made?	Conplementary sticky ends are made when the same restriction endonuclease is used to cut out the plasmid & the gene from the chromosome
What Does Ligase Do?	Ligase seals the gene into the plasmid
What Is A Vector?	A vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell
What Are Selectable Markers Used For In Vectors?	Selectable markers are present in the vector to ensure only mirco organisms that have taken up the vector grow in the presence of a selective agent (Antibiotic)
Name A Safety Precaution Used In Genetic Manipulation	Genes are often added to prevent survival of the micro organism in an external environment

Resource summary

	Created by Ellie Potellie over 5 years ago