Unit 1: DNA Replication & PCR

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Cfe Higher Cfe Higher | Biology (Unit 1: DNA & The Genome) Flashcards on Unit 1: DNA Replication & PCR, created by Ellie Potellie on 19/12/2018.
Ellie Potellie
Flashcards by Ellie Potellie, updated more than 1 year ago
Ellie Potellie
Created by Ellie Potellie almost 6 years ago
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Question Answer
What Enzyme Is Responsible For DNA Replication? DNA Polymerase
What Is Meant By Continuous Synthesis? Whole strand is made in one piece
What Is Meant By Dis-Continuous Synthesis? Strand is replicated in fragments
What Is The Function Of PCR? To amplify a sequence of DNA
Why Is DNA Heat Back Up For The Third Stage Of PCR? To allow taq polymerase to synthesise the new strands of DNA
Why Is DNA Cooled During The Second Stage Of PCR? To allow primers to anneal to the template strands
Why Is Taq Polymerase Used In PCR? It doesn't denature during heating cycles
Which Rule Allows The Molecules of DNA To Be Copied? Complementary base pair rule
What Temperature Does The Second Stage Of PCR Occur At? 55^* and 65^* (Any Within Range)
What Temperature Does The First Stage Of PCR Occur At? 94^* & 96^* (Any Within Range)
Why Is DNA Heated In The First Stage Of PCR? To break the hydrogen bonds between the bases and separate the two strands
Which Bonds Are Broken To Separate Two Strands Of DNA? Hydrogen Bonding
Which Enzyme Joins The Fragments Of DNA On The Logging Strand? Ligase
What Is The Difference Between DNA Polymerase and Taq Polymerase? DNA Polymerase denatures at lower temperatures than taq polymerase
When Is PCR Used? Crime scene analysis to identify suspects, paternity testing, identifying evolutionary relationships
What Are The Requirements Of PCR? •Template DNA •Taq Polymerase •Primers •Free DNA nucleotides •Buffer
Why Is DNA Replication Required? Before mitosis to ensure that both daughter cells get an identical copy of the genetic information from the parent cell
Why Is There a Leading and Lagging Strand In DNA Replication? Parent strands are anti-parallel but DNA can only by synthesised in 5 - 9 direction
Which Strand In DNA Replication Requires The Use Of Many Primers? Lagging Strand
Why Are Two Different Primers Needed During PCR? One which is complementary to each strand being copied
What Is The Function Of A Primer? To Provide a start point for DNA polymerase
Why Can DNA Only Be Replicated In One Direction? Because DNA Polymerase can only add nucleotides to the 3" end
What Temperature Does The Third Stage Of PCR Occur At? 72^*
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