3294787
Description
Flashcards by harrisoncr, updated more than 1 year ago
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Created by harrisoncr
over 8 years ago
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| Question | Answer |
| Define a bacterial colony. | Visible cluster of microorganisms growing on the surface of or within a solid medium, presumably cultured from a single cell. |
| List five characteristics that are used to describe colonial morphology. | 1) Color 2) Density 3) Consistency 4) Surface texture 5) Shape and size |
| Explain why it is important to obtain pure cultures of bacteria. | To be able to study the properties of individual species without interference from other species. |
| Describe three forms of culture media and the advantages and disadvantages of each form. | 1) Solid: Advantages-isolating and separating bacteria growing together in a specimen; Disadvantages-Increases chances of mixed cultures. 2) Liquid: Advantages-Understanding the organisms oxygen requirements; Disadvantages-Mixed organisms can't be separated. |
| List three aseptic techniques used in the laboratory to prevent contamination of the worker, the environment, and culture material. | 1) Handwashing 2) Disinfecting lab benches 3) Tying back long hair. |
| How would you determine whether culture media given to you are sterile before you use them? | Place them in the incubator without removing the cap/lid. |
| What are the signs of growth in a liquid medium? | -Turbid and diffuse -Layered at surface only -Layered below surface; none beneath center -Forms puff balls, layered below surface |
| What is the purpose of wiping the laboratory bench top with disinfectant before you begin to handle cultures? | Helps to prevent contamination. |
| Why can a single colony on a plate be used to start a pure culture? | Can be used as a control for comparing other bacteria and reduce contamination. |
| Why is it important not to contaminate a pure culture? | To be able to correctly identify the bacteria. |
| What is meant by the term clonal morphology? | Shape, size, consistency, surface texture, elevation, color, and density |
| Why should long hair be tied back when one is working in a microbiology laboratory? Can you think of an actual patient-care situation that would call for its control for the same reason? | -To not contaminate the plates |
| Name at least two kinds of solutions that may be administered to patients by intravenous injection and therefore must be sterile. How would you know if they were not sterile? | -Solutions: Crystalloid and colloid -Suspicious if solution is cloudy or if the integrity of the packaging has been compromised |
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