495869
Description
Flashcards by sophietevans, updated more than 1 year ago
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Created by sophietevans
over 10 years ago
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| Question | Answer |
| Which neurotransmitter was used to stimulate the muscle tissue? | Acetylcholine. |
| What type of muscle tissue was used? | Pig bladder detrusor smooth muscle tissue. |
| What type of relationship exists between the muscle activity and the concentration of the drug added? | A quantitative one, if not a proportional one. |
| Which piece of technical equipment was the force transducer attached to? | The PowerLab panel. |
| Which piece of software was used in the lab? | LabChart |
| What units is the raw output of the force transducer measured in? What did you want the units to be? | The raw output is in mV, but the more meaningful units that we wanted to establish were grams (of force that the muscle contracted with). |
| How heavy was the weight used to calibrate the force transducer? | 1g |
| What solution was the tissue kept in? Why was this? | Ringer Tyrode is a physiological solution of salts isotonic with the tissue that maintains appropriate osmotic balance to keep the tissue alive. |
| What were the relative proportions of O2 and CO2 provided to the tissue? At what volume was this provided? | 95% O2 and 5% CO2, provided at a volume of 1 bubble per second. |
| If 1ml of 1 x 10^-5M acetylcholine solution is added to 9ml of physiological solution, what is the final tissue bath concentration of drug that the tissue experiences? | 1 x 10^-6 M, because the Ringer Tyrode dilutes it further. |
| The bladder detrusor muscle took longer to plateau (maximal force exerted by maximal contraction at that concentration of acetylcholine) than the rat intestinal smooth muscle in the last organ bath. How is this related to its function? | The plateau took longer to reach because there are fewer receptors in the bladder for ACh than in intestinal tissue. The bladder is not only involved in the excretion of urine, but also in its storage and concentration, so it is important that the tissue is not hyper-excitable so that it can carry out this function. On the other hand, frequent peristalsis is important in the digestion and absorption of food in the intestines, and so an easier/earlier plateau is more appropriate. |
| What would you expect to happen to the response (in grams) as the concentration of acetylcholine increased? | It would increase proportionally, as more acetylcholine bound with receptors and resulted in smooth muscle contraction. |
| Given that the atropine and acetylcholine powder were not in contact with students, what was the biggest risk in this lab? | The pig tissue could have been a source of infection if not handled with gloves and disposed of correctly (given to member of staff, who put it in the macerator). |
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