According to the WHO, the viral
outbreak has led to 2240 suspected
and con- firmed cases and 1229
deaths in four countries: Guinea,
Liberia, Nigeria, and Sierra Leone
Family: Filoviridae
OTher Categories
Target
Humans besides Zaire
Bundibugyo virus
Sudan virus
Taï Forest virus
Non-Human Primates
Reston virus
Identified in 1976 and
named after the Ebola
River where the first
known epidemic occurred
References:
Zhang YF, Li DP, Jin X, Huang Z.
Fighting Ebola with ZMapp: spotlight
on plant-made antibody. Sci China
Life Sci, 2014, 57: 1–3, doi:
10.1007/s11427-014-4746-7
Endothelial Cells in Physiology and in the Pathophysiology of
Vascular Disorders in
http://www.bloodjournal.org/content/91/10/3527?sso-checked=true.
Institute for Molecular Bioscience, University of
Queensland, Q4072, Australia.
D.Hume@imb.uq.edu.au. The MPS in
http://www.ncbi.nlm.nih.gov/pubmed/16338128.
Hepatic Histology in
http://www.vivo.colostate.edu/hbooks/pathphys/digestion/liver/histo_hcytes.html
Seasonal Influenza
Infection Control
Guidelines 2010
CDC in
http://www.cdc.gov/flu/professionals/infectioncontrol/index.htm
Monoclonal antibody drugs for cancer in
http://www.mayoclinic.org/diseases-conditions/cancer/in-depth/monoclonal-antibody/art-20047808
Fatality Rate: 90%
the proportion of deaths
within a designated
population of "cases"
Infection
Steps of infection
Step 1
Endothelial Cells
ECs line vessels in every organ system
and regulate the flow of nutrient
substances, diverse biologically active
molecules, and the blood cells
themselves.
Mononuclear Phagocytes
Defined as a family of cells comprising
bone marrow progenitors, blood monocytes
and tissue macrophages. Macrophages are
a major cell population in most of the
tissues in the body.
Hepatocytes
Cells of the liver that perform
an astonishing number of
metabolic, endocrine and
secretory functions
Step 2
Evade the host immune
system
Step 3
Damages internal tissues and organs,
such as blood vessels and the liver: ultimately
death.
Transmission
Contact with Body Fluids
Aerosol infection not
proved clinically
Droplets: land directly on mucosal lining of nose,
mouth, eyes of nearby persons or can be inhaled.
Highest exposures within 3-6 feet.
Airborne: aerosols become smaller by evaporation;
small aerosols (≤ 10 microns) remain suspended for
longer periods, if inhaled travel deep into the lungs.
Experimental drug co-developed by Mapp
Biophamaceutical, Inc. (San Diego, CA, USA)
and Defyrus 2 Zhang. (Toronto,
Canada).
Combination of three “humanized” monoclonal
antibodies (mAbs) against the EBOV GP protein.
Monoclonal antibodies mimic the
antibodies your body naturally
produces as part of your immune
system's response to germs, vaccines
and other invaders.
Produced in plant: 3
MAbs produced in these glycomodified
plants had mammalian-like glycans with
more homogeneous glycoforms than that
produced in CHO cells
Glycans: large number of monosaccharides linked glycosidically
History
Plant made antibody studies
First Wave: Production of
monoclonal antibodies (1989)
Transgenic Technology
Delivery of genes of interest into plant cells,
subsequent integration of theses genes into the
parental plant chromosome, and selection of
stable transgenic plants.
Stopped by a number of issues associated with this expression
strategy, including low expression levels, long timeframes to
generate transgenic plants, undesired glycosylation patterns, and
concerns on spreading transgenes into the environment
Second Wave: replica- tion-competent viral
vectors, such as magnICON and Gemini
These vectors contain elements required for viral replicon formation. Once delivered into plant cells via
Agrobacterium infiltration, these vectors are able to assem- ble into replicons, which undergo automatic
replication and result in a high copy number of RNA molecules encoding the desired antibody.