Arron Watson
Mind Map by Arron Watson, updated more than 1 year ago
Arron Watson
Created by Arron Watson over 6 years ago


covering some of the basic topics found in genetics

Resource summary

  1. History
    1. Gregor Mendel - Pea plants 1866
      1. Johann Friedrich Miescher DNA
      2. deoxyribonucleic acid
        1. nuclein was rich in phosphorus and was acidic
          1. Nucleases are enzymes that act on nucleic acids
            1. DNA / RNA
              1. polymers made up of monomer s callednucleotides
                1. DNA/RNA polymers made up of monomers called nucleotides
                  1. Five-carbon-ring sugar (deoxyribose or ribose) Phosphate group Nitrogen base
                    1. Nitrogen Bases Differences
                      1. RNA
                        1. Uracil
                        2. DNA
                          1. Thymine (T for two bonds/ G for three)
                          2. Both have - Adenine - Cytosine - Guanine
                  2. Molecular Genetics
                    1. Gene Expression
                      1. Antibodies bind to specific foreign particles, such as viruses and bacteria, to help protect the body. Immunoglobulin G (IgG) Example of expression for protein
                        1. 20 different types of amino acids
                          1. transcription and translation = Gene expression
                            1. Messenger RNA
                              1. Transfer RNA
                                1. Start and stop codons
                                  1. AUG (STOP) Methionine
                                    1. UUG / GUG (START) Valine / luecine
                          2. Genetic code
                            1. UGG - Tryptophan
                              1. UGA - Opal
                                1. UGU - Cysteine
                                  1. ALL RNA
                                  2. DNA code table U replaced by T
                                  3. Genetic engingeering
                                    1. Dna inserted into host genome (Commonly a phage)
                                      1. Phage (Bateriophage) cell that attacks bacteria
                                        1. Isolating and copying genetic material
                                          1. Molecular cloning
                                        2. Gene targeting
                                          1. Delete and add genes / remove exons
                                            1. = Homolougous recombination
                                              1. point mutations
                                            2. GM - Mice (oncomouse ) used for many experiments - Cancer - diabetes
                                              1. Gene therapy
                                                1. Replacing defective genes in humans ( Germline gene therapy )
                                                  1. Somatic gene therapy - used for Parkinsons disease
                                                2. Cloning of any DNA fragment essentially involves four steps 1 fragmentation - breaking apart a strand of DNA 2 ligation - gluing together pieces of DNA in a desired sequence 3 transfection - inserting the newly formed pieces of DNA into 4 cells screening/selection - selecting out the cells that were successfully transfected with the new DNA
                                              2. The basics
                                                1. Genes
                                                  1. Total genes in an organism = Genome (can be multiple sites)
                                                    1. Each gene is located on the chromosome = a locus (site)
                                                    2. 23 pairs = 46 chromsomes = one human
                                                      1. 22 Automsomes in both female and male -
                                                        1. XX in Females (23rd pair differ)
                                                          1. XY in Males (23rd pair differs)
                                                            1. More SRY (sex-determining region Y) means male offspring
                                                          2. Mitosis and mieosis
                                                        2. Transcription
                                                          1. Translation
                                                            1. Similar to transcription but no replication of DNA instead = rRNA
                                                              1. tRNA is used as well
                                                                1. Location: Nucleus in transcription - cytoplasm in translation
                                                                  1. Terminator
                                                                    1. Three top stop codons cause this
                                                            2. mRNA copies the DNA message
                                                              1. mRNA (messenger RNA) takes the message to the nucleus
                                                                1. The steps; get ready to transcribe, enzymes find right DNA. DNA is opened up (initiation) followed by the building of mRNA (elongation) then the DNA molecules snap shut to release the mRNA (termination)
                                                                  1. Promoter
                                                                    1. Terminator
                                                                      1. RNA transcript causes this
                                                              2. Technology to manipulate DNA
                                                                1. PCR ( polymerase chain reaction )
                                                                  1. Amplification from genomic DNA templates
                                                                    1. Thermocycler (amplification)
                                                                      1. Repeated heating and cooling (denaturation)
                                                                        1. Heating seperates
                                                                          1. Cooling binds
                                                                      2. Gel electrophoresis
                                                                        1. Agrose gel
                                                                          1. Seperated by weight
                                                                        2. Annealing step (binding process)
                                                                          1. Elongation step (Enzymatic replication / DNA polymerase)
                                                                            1. PCR then acts as a chain reaction and the DNA template is repeatedly amplified and replicated
                                                                      3. Recombination
                                                                        1. Plasmids/phage used
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