CELL CULTURE

Olaia Gorriz
Mind Map by Olaia Gorriz, updated more than 1 year ago
Olaia Gorriz
Created by Olaia Gorriz about 5 years ago
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CELULA
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CELL CULTURE
1 PREPARATION OF MEDIUM FORMS
1.1 CONCENTRATE
1.2 WORKING
1.3 POWERED
2 COMPOSITION AND PROPERTIES OF THE CULTURE MEDIUM
2.1 MINERAL SALS
2.2 GLUCOSE
2.3 VITAMINS
2.4 TAMPON
2.5 ORGANICS SUPLEMENTS, LOW MOLECULAR WEIGHT
2.6 HORMONES GROWTH FACTORS
2.6.1 = ANIMALS SERUM
2.6.1.1 + PROTEINES
2.7 ANTIBIOTICS ANTIFUNGALS
3 CRITERIA FOR SELECTING MEDIA
3.1 CELL LINES
3.1.1 THE CHOICE OF CELL CULTURE MEDIAL IS EXTREMELLY IMPORTANT & SIGNIFICANTLY AFFECTS THE SUCCES OF CELL CULTURE EXPERIMENS
3.2 PRIMARY CELL CULTURE
3.2.1 PRIVIDES UNIQUE & VALUABLE RESEARCH DATA
3.2.2 COMPLETE & CONDICIONED MEDIUM
3.2.2.1 REDUCES
3.2.2.1.1 RISK OF CONTAMINATION
3.2.2.1.2 TIME
3.2.2.1.3 LABOR
3.2.2.1.4 MONEY
4 COMMON CELL CULTURE MEDIA
4.1 EAGLE´S MINIUM ESSENTIAL MEDIUM (EMEM)
4.1.1 CONTAINS
4.1.1.1 SODIUM PYRUVATE
4.1.1.2 NONESSENTIAL AMINO ACIDS
4.1.1.3 SAL SOLUTION
4.1.2 NON-COMPLEX MEDIUM
4.2 RPMI-1640
4.2.1 GENERAL PURPOSE MEDIA
4.2.1.1 MAMMALIAN CELSS
4.2.1.1.1 HEMATOPOIETIC CELSS
4.2.1.1.2 HUMAN LYMPHOCITIES FUSION PROTOCOLS CROWTH OF HYBRID CELLS
4.3 DUBLECCO´S MODIFIED EAGLE´S MEDIUM (DMEM)
4.3.1 CONCENTRATIOM
4.3.1.1 AMINO ACIDS
4.3.1.2 VITAMINES
4.3.2 BASAL MEDIUM
4.3.2.1 WITH SUMPLEMENTATION
4.3.2.1.1 COMPLETE MEDIUM
4.4 DMEM/F-12
4.4.1 MIXTURE OF DMEM & HAM´S F-12
4.4.1.1 RICH MEDIUM
4.4.1.1.1 COMPLEX MEDIUM
4.4.1.2 CELLS TYPES IN BOTH SERUM
4.5 ISCOVE´S MODIFIED DULBECCO´S MEDIUM (IMDM)
4.5.1 CONTAINING
4.5.1.1 SELENIUM
4.5.1.2 AMINO ACIDS
4.5.1.3 VITAMINS
4.5.1.4 INORGANIC SALT
4.5.1.5 HEPES
4.5.1.6 POTASIIUM NITRATE
4.5.1.7 SODIUM PYRUVATE
4.5.2 GROWTH OF LYMPHOCYTES & BYBRIDOMAS
4.6 HAM´S NUTRIENT MIXTURES
4.6.1 F-10 HAM
4.6.1.1 GROWTH OF HUMAN DIPLOID CELLS & WHITE BOOD CELLS
4.6.2 F-12 HAM
4.6.2.1 GROWTH OF PRIMARY RAT HEPATOCITES RAT PROSTATE EPITHELIAL CELSS
4.6.3 COON F-12 HAM
4.6.3.1 CULTURING HYBRID CELLS
4.6.3.2 CONTAIN
4.6.3.2.1 DOBLE AMINO ACIDS & PIRUVATE
4.6.3.2.1.1 F-12 HAM
4.6.3.2.2 ASCORBIC ACID
5 INCUBATION CONDITIONS
5.1 Tª
5.1.1 28Cº INSECTS 35Cº ANIMALS
5.2 HUMIDITY 95%
6 FEATURES PHYSIOCHEMICAL CULTURE MEDIUM
6.1 PH 7,2-7.6
6.2 OSMOLARITY 200-320 OSM/KG
6.3 VISCOSITY
6.4 SURFACE TENSION
7 GAS ATMOSPHERE
7.1 WERE GAS MIX CULTURES ARE MAINTAINED
7.2 COMPONENTS
7.2.1 OXYGEN
7.2.2 CO2
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