SPECIMEN PREP, CULTURE, & HARVEST

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tsh2v
Created by tsh2v almost 6 years ago
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SPECIMEN PREP, CULTURE, & HARVEST
1 SPECIMEN HANDLING
1.1 SAMPLE TREATMENT
1.1.1 ANTI- BIOTICS/FUNGICIDES
1.1.1.1 Penicillin/ Streptomycin
1.1.1.1.1 bacterial
1.1.1.2 Kanamycin
1.1.1.2.1 bacterial
1.1.1.2.2 > Pen/Strept
1.1.1.3 Gentamycin
1.1.1.3.1 yeast
1.1.1.3.2 bacterial
1.1.1.4 Mycostatin/Amphotercyn
1.1.1.4.1 fungal
1.2 SAMPLE TRANSPORT CONDITIONS
1.2.1 PERIPHERAL BLOOD
1.2.1.1 PHA
1.2.1.1.1 division stimulated
1.2.1.2 1-2 days
1.2.2 BM
1.2.2.1 divides constantly
1.2.2.2 Sodium Hep
1.2.2.3 >4hrs
1.2.2.3.1 add media
1.2.2.3.1.1 room temp.
1.2.3 SOLID TISSUES
1.3
1.4
2 CULTURING TECHNIQUES
3 HARVESTING TECHNIQUES

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