Flashcards
Sem título
Flashcards do conjunto (50)
- 1What should be done with the cap of the test tubes?Leave it loosened for adequate aeration
- 2How are the acetate agar tubes inoculated?By streaking the surface of the slant
- 3What is the procedure for the experiment?Dispensing sterile saline or suitable broth into a small test tube
- 4What is the first step in preparing the suspension?Shake by hand or on a vortex
- 5What is the purpose of the catalase test?Detect the ability of organisms to produce the catalase enzyme
- 6How long are the tubes incubated for?Up to 7 days
- 7What is the principle behind using bacitracin in differentiation?Bacitracin inhibits bacterial cell wall synthesis
- 8How long should the tubes be incubated for?Three hours
- 9What does a positive result in the catalase test indicate?Catalase-positive micrococcal and staphylococcal species
- 10What is the expected result for a positive test?Blue color change (green to blue)
- 11What is the distance between the antibiotic disks?About 10 mm
- 12What is the purpose of the butyrate disk test?To detect the enzyme butyrate esterase and aid identification of Moraxella catarrhalis.
- 13What does a negative result in the catalase test indicate?Catalase-negative streptococcal species
- 14What is the expected result for a negative test?No color change (remains green)
- 15What is done to ensure adherence of the disks to the plates?Tapping with a sterile stick
- 16What are the expected results of the butyrate disk test?+ Lysis of colonies or clearing (positive), - Intact colonies (negative)
- 17What is the principle of the catalase test?Catalase converts hydrogen peroxide to water and oxygen
- 18What is the procedure for acetate utilization?Take a well-isolated colony from an 18 to 24-hour culture with a sterile inoculating needle.
- 19What temperature are the plates incubated at?35-37°C
- 20What is the expected result for reagent bromo-chloro-indolylbutyrat?Development of a blue color.
- 21What is the purpose of BILE ESCULIN AGAR test?To identify and differentiate members of Enterococci and Group D Streptococci from other viridans or non-Group D Streptococci.
- 22What is the principle of BILE ESCULIN AGAR test?Bile salts inhibit gram (+) organisms and it selects organisms based on their ability to grow in a medium with 4% bile salts followed by their ability to hydrolyze esculin to esculetin.
- 23What are the expected results for BILE ESCULIN AGAR test?Positive: Growth and blackening of the agar slant caused by ferric citrate; Negative: Growth and no blackening of medium.
- 24What is the purpose of BILE SOLUBILITY TEST?To differentiate Streptococcus pneumoniae (positive–soluble) from alpha-hemolytic streptococci (negative–insoluble).
- 25What is the purpose of the CAMP test?Differentiate group B streptococci (Streptococcus agalactiae - positive) from other β-hemolytic streptococci
- 26What is the purpose of acetamide utilization?Differentiate microorganisms based on the ability to use acetamide as the sole source of carbon.
- 27Should the cap of the test tubes be left loose or tightened?Left loosened for adequate aeration
- 28What is the function of sodium desoxycholate in the principle?Faster lysis or clearing
- 29What should be done after preparing the suspension?Divide into two tubes
- 30How should the unknown microorganism be streaked?Perpendicular to S. aureus without touching it.
- 31What is the first step in the procedure?Taking a well-isolated colony with a sterile inoculating needle
- 32What is the recommended incubation temperature for the tubes?35-37oC
- 33What is the role of amidase in Diplococcus?Natural autolytic enzyme
- 34What can be used to examine the tubes during incubation?Gram stain or methylene blue wet mount
- 35Where should the positive control organism be streaked?Parallel to the unknown organism, but not touching it.
- 36How are the acetamide agar tubes inoculated?By streaking the surface of the slant
- 37What is the purpose of conducting a Bacitracin susceptibility test?To determine if a bacterium is susceptible or resistant to Bacitracin.
- 38What happens when sodium desoxycholate is added to Diplococcus?Catalyzes the reaction
- 39What is the principle behind the butyrate disk test?Organisms capable of producing butyrate esterase hydrolyze bromochlorindolyl butyrate, releasing indigo.
- 40What is the purpose of acetate utilization?Differentiate organisms based on ability to use acetate as the sole source of carbon.
- 41What happens to the growth of bacteria when peptidoglycan production is inhibited?No growth occurs.
- 42What is the result if amidase is not present?No clearing
- 43What is the principle behind acetate utilization?Organisms capable of using sodium acetate grow on the medium, resulting in an alkaline pH, turning the indicator from green to blue.
- 44Which specific bacterium is inhibited by Bacitracin?Group A β-hemolytic Streptococci (S. pyogenes).
- 45How is bacitracin used to differentiate organisms?A bacitracin disk is placed on an agar plate and zones of inhibition are observed
- 46What is the principle behind acetamide utilization?Bacteria capable of growth on this medium produce the enzyme acylamidase, which deaminates acetamide to release ammonia. The production of ammonia results in an alkaline pH, causing the medium to change color from green to royal blue.
- 47What is the first step of the procedure?Preparing bacterial suspension from overnight culture
- 48How long should the test tubes be examined before discarding the result as negative?4 days and again at 7 days
- 49How are the BAP/MHA plates inoculated?With sterile swabs to form bacterial lawns
- 50How long are the inverted plates incubated?24 hours
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- FlashCards sobre Sem título, criado por Mikaela Evangelista em 11-11-2023.
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- Mikaela Evangelista
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