Flashcards

Paper Chromatography

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1ChromatographyMethod used to separate substances in a mixture. Can be then used to identify substances.
2Mobile PhaseWhere molecules can move. This is always in a liquid or a gas,
3Stationary PhaseWhere molecules can't move. This can be in a solid or really thick liquid.
4What happens in chromatography?1. filter paper stationary phases
2. draw a pencil line 1cm from the bottom
3. sample of the unknown substance is spotted
3. filter paper placed in a small amount of solvent
4. solvents the mobile phase
5. components of the mixture dissolve in the solvent and travel up the filter paper
6.the resulting pattern is called the chromatogram
7. dyes move up the paper separate and create series of blogs
5what 2 things affects the amount of time the molecules spend in each phase?1. solubility in the solvent
2. attraction to the paper
6What is a Rf value?The travelled distance by the dissolved substance divided by the distance travelled by the solvent.
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