Flashcards
BioChem Exam 1
Flashcards do conjunto (16)
- 1if the H ion concentration of the blood increases 10-fold, how much does the pH change?lower by 1 pH unit
- 2the side chain of valine & isoleucine can form ____ bonds w/ each otherhydrophobic
(both non-polar) - 3organic carboxyl groups generally function as:weak acids
- 4a purification method which depends on highly selective interactions between a protein's binding site and a specific ligantaffinity chromatography
- 5what type of protein would stock very tightly to a positively charged DEAE-cellulose exchange collumn?a protein that was highly negatively charged
- 6The migration of protein subunits in a SDS-polyacrylamide gel electrophoresis system depends mostly on:the size of the native protein subunit
- 7enzymes canchange the rate of a reaction
- 8what is the primary reagent used to sequence proteins during the Edman degredationphenylisothiocyanate
- 9what reagent is used to prevent reoxidation of sulfylhydryl groups during the reduction of protein disulfidesiodoacetate
- 10the phi and psi bonds that flank peptide bonds in proteins:are able to rotate but generally assume certain combinations of bond angles
- 11the overall free energy change for a chemical reaction is positive if the reaction:does not favor product formation
- 12vitamins are:enzymes cofactors or components of enzyme cofactors
- 13Biochemists use a modified form of the standard free energy because:enzyme reaction rates usually take place around pH=7
- 14the factor which measures the number of molecules of substrate reacted per enzyme active site and which does not change as an enzyme is purifiedturnover number
- 15which of the following is NOT true about the ATP cofactorit is often hydrolyzed to adenosine to promote enzyme reaction
- 16chemical reactions and enzyme reactionsvary in rate but ultimately can produce the same level of product
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- FlashCards sobre BioChem Exam 1, criado por halle074 em 13-02-2015.
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