Flashcards
Cells, Tissues, Organs & Development MIDTERM
Flashcards do conjunto (15)
- 1What dye, used in histology, has a net positive charge and binds to components of cells and tissues that are negatively charged?Basic Dyes
- 2What components of cells and tissues are negatively charged and stain with basic dyes?~ Phosphate groups of nucleic acids (DNA and RNA)
~ Sulphate groups of some polysaccharides (glycosaminoglycans) and some proteins (mucus) - 3What are tissue components that stain with basic dyes referred to?Basophilic
- 4What dye, used in histology, has a net negative charge and binds to components of cells and tissues that are positively charged?Acidic Dyes
- 5What components of cells and tissues are positively charged and stain with acidic dyes?~ Ionized amino groups in proteins (side chains of lysine and arginine)
- 6What are tissue components that stain with acidic dyes referred to?Acidophilic
- 7What is a positively charged, blue dye complex that stains with high affinity basophilic structures?Hematoxylin
- 8What is most intensely stained when stained with hematoxylin?Nuclei
- 9Why are nuclei basophilic?Hematoxylin bind to negatively charged complexes of condensed DNA and histones (i.e., heterochromatin)
- 10What is a negatively charged, pink dye that stained with high affinity acidophilic structures?Eosin
- 11What is most intensely stained when stained with eosin?Cytoplasm
- 12Why is the cytoplasm eosinophilic?Eosin binds to positively charged proteins in the cytoplasm
- 13What is the most commonly used technique in histology?Hematoxylin and Eosin (H&E)
- 14What structures do not stain well with hematoxylin and eosin?Hydrophobic structures (remain clear)
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- FlashCards sobre Cells, Tissues, Organs & Development MIDTERM, criado por Caitlin McCarthy em 24-10-2023.
- Criado por
- Caitlin McCarthy
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