Flashcards
Minimum Genome
Flashcards do conjunto (29)
- 1GenomeEntire Genetic Compliment of a Genome
- 2C-ValueTotal amount of DNA in the haploid genome
- 3C-Value paradoxLack of correlation between C-value and complexity due to various levels of repeating DNA
- 4Viral Genome Size Variation10^3 - 10^6
- 5Bacterial Genome Size Variation10^6-7
- 6Eukaryotic Genome Size Variation10^6-11
- 7Oganism In Which C-Value Is Related To ComplexityBacteria
- 8Mycoplasma GenitaliumSmallest simple parasite
- 9Nasuia DeltocypheriaSmallest bacteria known- 112,000kbp
- 10Genome size is _ proportional to gene numberDirectly
- 11Pelagibacter Ubique- Genome Size1 million kBp (approx)
- 12Pelagibacter Ubique- Why so important?Smallest free living organism
- 13Pelagibacter Ubique- How so small?Few regulatory gene & no pseudo/phage genes
- 14Viral Overlapping GenesUsually each strand codes a separate gene i.e. VP3 is found in coding region for VP2
- 15Bacterial RepliconsMegaplasmids/minichromosomes
- 16Bacterial Chromosomes1- few have more
- 17Bacterial Chromosome Replication- BeginsAt origin- though there can be multiple origins
- 18Genes Near OriginTend to be more important
- 19Bacterial Gene OrientationIn direction of replication- prevents head on collision between replication fork & transcription factors
- 20OperonGroup of genes transcribed onto a single mRNA
- 21ClusterGenes found together & involved in the same process but transcribed separately
- 22Experimentation- Null MutationsSee what effect this has on organism (done through recombination/RNA interference)
- 23Recombination Cauing Null MutationsIntroduce gene containing a marker halfway through gene to be disrupted
- 24Minimum GenomeTheoretical minimum no of genes needed to sustain life
- 25Essential genesRequired for core processes i.e. DNA replication
- 26Non-essential genesNot vital but incur an advantage on organism
- 27Finding Core Genes- MethodsNull mutations/DNA comparisons
- 28Finding Core Genes- DNA comparisonsCompare two small genomes- genes in common are most likely to be vital
- 29Finding Core Genes- Null MutationsStudy effects of null mutations- if organism dies, gene is most likely to be vital
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- Year 2 FlashCards sobre Minimum Genome, criado por gina_evans0312 em 28-12-2013.
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- gina_evans0312
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