Fluxograma
Disaggregation of Explant
Conteúdo do fluxograma
DISAGGREGATION OF EXPLANTS
Primary explant technique
Mechanical diasaggregation
Dissection of explant
Finely chopped
Washed by setting
Transfer in last wash
REmove BSS
Add thin flim of medium
Explant out growth
Incubate 2 to 3 weeks
TISSUE SAMPLE
CELL+DEBRIS
MORE CELLS+DEBRIS
VIABEL CELLS+DEBRIS
DILUTE THE CELLS
INCUBATE
Enzymatic disaggregation
Biopsy in medium+antibiotic
Dissection in BSS+antibiotic
Chopped tissue
Complete medium+
collagenase
Dissagregated tissue+
tissue residues
Dispersed cells+residual culture of epithelial cells
Dispersed cell suspension contains enriched fibrobiasts
Enriched epithelial fraction
Primary cell culture
Informações
- Descrição
- Fluxograma sobre Disaggregation of Explant, criado por vallarasu VîP em 27-12-2017.
- Criado por
- vallarasu VîP
- Visualizações
- 34
- Avaliação