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Flowchart

Disaggregation of Explant

vallarasu VîP English 5.0Average rating 5.0 out of 5 (1 rating)

Flowchart content

  1. DISAGGREGATION OF EXPLANTS

  2. Primary explant technique

  3. Mechanical diasaggregation

  4. Dissection of explant

  5. Finely chopped

  6. Washed by setting

  7. Transfer in last wash

  8. REmove BSS

  9. Add thin flim of medium

  10. Explant out growth

  11. Incubate 2 to 3 weeks

  12. TISSUE SAMPLE

  13. CELL+DEBRIS

  14. MORE CELLS+DEBRIS

  15. VIABEL CELLS+DEBRIS

  16. DILUTE THE CELLS

  17. INCUBATE

  18. Enzymatic disaggregation

  19. Biopsy in medium+antibiotic

  20. Dissection in BSS+antibiotic

  21. Chopped tissue

  22. Complete medium+

    collagenase

  23. Dissagregated tissue+

    tissue residues

    ​​

  24. Dispersed cells+residual culture of epithelial cells​​​​​​

  25. Dispersed cell suspension contains enriched fibrobiasts

  26. Enriched epithelial fraction

  27. Primary cell cul​​​​​​ture

Information

Description
Flowchart on Disaggregation of Explant, created by vallarasu VîP on 27/12/2017.
Created by
vallarasu VîP
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Average rating 5.0 out of 5 (1 rating)

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