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1. Microbiological analysis of soil

2. Analysis of spread plates

3. Charactorising isolates &​​​​Preparing patch plates

4. Preparing overlay plates

5. Examining overlay patch plates and PCR

6. Gel electrophoresis & PCR product purification

Serial dilution

Spread plates in triplicates for undiluted sample &10-4

Selection of isolates

Streak plates

Gram & flagella stain

Catalase & oxidase test

Patch plate preparation

Preparation of overlay plates

Re-streak bacterial isolates

Examine zone of inhibiton

Preparation 9 PCR reactions

Gel electropheresis part 1

DNA purification

Gel electropheresis part 2