1033094
Descrição
Mapa Mental por elisemaybishop, atualizado more than 1 year ago
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Criado por elisemaybishop
quase 10 anos atrás
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Microscopy
- Magnification & Resolution
- Magnification is how much bigger it appears
under the microscope than it is in real life
- MAGNIFICATION = IMAGE SIZE + ACTUAL SIZE
- MAGNIFICATION = IMAGE SIZE + ACTUAL SIZE
- The term resolution refers to the ability to see
two distinct points separately
- Magnification is how much bigger it appears
under the microscope than it is in real life
- The light microscope
- Light passes from a bulb under the stage...
- ...through a condenser lens...
- ...and then through the specimen
- This beam of light is passed
through an objective lens and then
the eyepiece lens
- This beam of light is passed
through an objective lens and then
the eyepiece lens
- ...and then through the specimen
- ...through a condenser lens...
- OVERALL MAGNIFICATION = OBJECTIVE LENS
MAGNIFICATION x EYEPIECE LENS MAGNIFICATION
- Some specimens have to be prepared before
being viewed under the telescope
- 1. STAINING: coloured stains are chemicals that bind
to chemicals in the specimen that allow the specimen
to be seen
- 2. SECTIONING: specimens are
embedded in wax - thin sections are
then cut out without distorting the
specimen
- 1. STAINING: coloured stains are chemicals that bind
to chemicals in the specimen that allow the specimen
to be seen
- Some specimens have to be prepared before
being viewed under the telescope
- Light passes from a bulb under the stage...
- The Electron Microscope
- Higher resolution
- Generate a beam of electrons that can
distinguish objects at 0.2 nm apart
- Generate a beam of electrons that can
distinguish objects at 0.2 nm apart
- TRANSMISSION ELECTRON MICROSCOPE
- Electron beams pass through a very thin prepared sample and
electrons pass through denser parts less easily
- Electron beams pass through a very thin prepared sample and
electrons pass through denser parts less easily
- SCANNING ELECTRON MICROSCOPE
- The electron beam is directed onto a sample. The
electrons do not pass through the specimen, they
bounce off
- The electron beam is directed onto a sample. The
electrons do not pass through the specimen, they
bounce off
- Higher resolution
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