Protein Structure

Protein Structure

1. primary

   • order of AA
     • Sequencing (Insulin)
       • first: Feredrick Sanger
         • FDNB
         • HCL 6M
         • separating s-s bonds by reducing them
         • identify amino-terminal residue
       • dabsyl chloride dansyl chlrolide
         • instead of FDNB
         • fluorescent = easier
       • Edman degradation method
         • PTC
         • CF3COOH
         • recycling the remaining part sequencing again
       • 3 S-S bonds
         • 51 AA
   • peptide bonds
   • disulfide bonds

2. secondary

3. teritiary

4. quaternary

Imagens e arquivos do mapa