Chromatography

Chromatography

1. What is it?

   • A technique for the separation of a mixture by passing it in solution in which the components move at different rates.

2. How does it work?

   • 1) A ruler is used to draw a pencil line on the chromatography paper.
     • 2) A pipette is used to put a drop of the original coloured mixture on the pencil line.
       • 3) Some solvent is placed in a beaker and the chromatography paper is placed in the solvent.
         • 4) The solvent is allowed to rise up the paper which causes the substances in the original mixture to separate.
           • Rf = Distance moved by substance/ Distance moved by solvent

3. Results

   • A pure substance produces one spot on the chromatogram
   • An impure substance produces two or more spots

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