Why is genome synthesis difficult?

Chemical synthesis is limited to 100nt at a time

Manipulation of large fragments is difficult

What is essential when creating small fragments to be joined together later in clones?

The fragments cannot contain introns

The cell wall is treated with chemicals to make it permeable

The DNA is directly microinjected into the cell

The cell absorbs the DNA with other nutrients

To improve/redesign regulatory networks

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1Gene synthesis can be fully automated cheaply
1. AVerdadeiro
2. BFalso
2Why is genome synthesis difficult?
1. AChemical synthesis is limited to 100nt at a time
2. BManipulation of large fragments is difficult
3. CVectors are limited
3Synthesis of a genome must be done in small, overlapping parts
1. AVerdadeiro
2. BFalso
4How many kbpairs can PCR be used for?
1. A1-5
2. B5-10
3. C10-15
5What is essential when creating small fragments to be joined together later in clones?
1. AThe fragments must overlap
2. BThe fragments MUST be 7kb long
3. CThe fragments cannot contain introns
6When transplacting the synthetic genome between clones, what must not be present?
1. AProteins
2. BDNA
3. CRNA
7How is the receptor organism for the synthetic genome made able to receive it?
1. AThe cell wall is treated with chemicals to make it permeable
2. BThe DNA is directly microinjected into the cell
3. CThe cell absorbs the DNA with other nutrients
8Once the synthetic genome has been added to the cell, it divides as it contains two genomes
1. AVerdadeiro
2. BFalso
9Why synthetic organisms?
1. ATo see if we can
2. BTo improve/redesign regulatory networks
3. CTo produce protective genes
4. DBiofuels/biopharmecuticals

Descrição: Year 2 Quiz sobre Syntheetic Genomics, criado por gina_evans0312 em 28-12-2013.
Autor: gina_evans0312
Idioma: English
Visualizações: 500
Etiquetas: genetics and genomics genomes year 2
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