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Micro practical questions
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Questions in this quiz (68)
1what are the three organisms in this ?
- ASerritia marcecens
- Be. coli
- Cbacillus megaterium
- Dmicrococcus luteus
2what are some observations about this plate? what do you think this is ? what do you see where should it be incubated ?
- ASAB AGAR, media
- Bseveral varties of yeast colonies based on color
- Cyeast grows best at room temp on SAB media
- Dall are correct
- Enone are correct
3disinfection , did this work ?
- AYes you can see the presence of organisims only before desinfection but not after
- Bno you can see presence of organisms before and after
4match the broth properties to the correct description
[blank_start]1.[blank_end] Pellicle
[blank_start]2.[blank_end] Turbid
[blank_start]3.[blank_end] Sediment
[blank_start]4.[blank_end] Pigment
A.Cloudy
B Settling at bottom of tube
C. Color
D. Surface growth- A1.B
- B1.A
- C1.C
- D1.D
- E2. A
- F2.B
- G2.C
- H2.D
- I3.A
- J3.B
- K3.C
- L3.D
- M4.A
- N4.B
- O4.C
- P4.D
5Synder Agar measures the amount of [blank_start]Acid[blank_end] produced by the normal flora of the mouth in a medium containing sugar. The faster the production of [blank_start]Acid[blank_end] occurs the more susceptible a person may be to cavities also called [blank_start]Caries[blank_end].
- AAcid
- Bbase
- Csugar
- Dglucose
- EAcid
- Flactose
- Gglucose
- HCaries
- Ipellicle surface growth
6on the synder test what indicates a stonger likely hood of cavatives?
- Athe more yellow the agar has turned indicates more acidity
- Bthe greener the agar has turned indicates more acid that will be classified as a cavity
7what stain uses the following
primary stain -crysatal violet
mordrant-idiodine
decolorizer- acid alchol
counter stain -safrainin- Agram stain
- Bendospore
- Cacid fast
8what color do gram positive cells remain what color ?
- Apurple
- Bpink
- Ccolorless
9acid fast stain do no stain to the waxy walls and appear red or pink . which of the following is besat for acid fast staining ?
- Amyobacterium & nocardia
- Bm. tuberculosais
- CNocardia
- Dall are correct
10why isnt the gram stain used to stain the mycobacterium ?
- Athe waxy cell wall wont penetrate easy !
- Bit is possible we just dont do it that way
11endospore stain include bacillus & clostridium , and stain green which of the following is included in this procedure ?
- Amalachite green
- Bmalachinte blue
- Csafranin
- Diodine
12IDENTIFY THE MORPHOLOGY IN THE IMAGE BELOW
- ABacillus rod
- Bspirochete
- Cvibirio
- Dstaphyylococcus
13what structures are showing in the image to the right ?
- Afilaments
- Bhyphae
- Cbulbs/ sporangium
- Dbacuillus megaterium
14what type of microorgansim has these structures?
Filaments/hyphae
buls/ sporanguim- Afungi
- Balgae
- Carchea
15Is fungi eukaryotic or prokaryotic ?
- AEukaryotic
- BPro
16the synder test measures what ?
- Athe amount of acidity in the mouth produced by normal flora of the mouth
- Bthe amount of trapped gas in the mouth
17how are caries created ? which the BEST anwser
- As. mutants break down sucrose into fructose and attracting other organisms to produce lactic acid which then dissolves the calcium in teeth
- Bneglecting to brush teeth and poor diet
18which is gram + and which is Gram -
- Agram postive
- Bgram negative
19The permeability theory proposes that bacteria stain differently because of a chemical and structural difference in the cell wall
- ATrue
- BFalse
20what is the purpose of the ethyl alcohol in gram staining ?
- Aits a modrant
- Bdecolorizer
- Ccounterstain
21decolorizer used in gram stain is which of the following >?
- Aacid alchol
- Bethyl alcohol
22what decolorizer is used in the acid stain procedure ?
- Aacid alcohol
- Bethyl alcohol
23acid alcohol is necessary because zeihl neelson is not very soluble in order to properly decolorize in acid fast staining acid alcohol is required .
- ATrue
- BFalse
24what is the genus that shows an acid fast bacterial ?
- Amycobacterium
- Barchea
25what is structurally different that bacterium that necessitates the use if this staining procedure ?
- Amycolic acid on cell membrane
- Bmycolic acid on cell wall
26the raised wrinkled colonies would differentiate the colonies of mycobacterium on an agar plate
- ATrue
- BFalse
27the enzyme lysozyme causes cell to lyse
- ATrue
- BFalse
28where on our body can we find lysozyme?
- Ablood
- Bsweat
- Ctears
- Dsalivia
29what type of stain is this >
- Acapsule
30what stains are used for capsule stains ?
- Acongo red
- Bethyl alcohol
- Cacid alcohol
- Dcarbol fuchsin
31disinfectants are used to sterlize
- ATrue
- BFalse
32Bacterial spores are much more resistant to heat, chemicals, irradiation and desiccation than their vegetative cell counterparts. The main food poisoning spore-formers are Clostridium botulinum, C. perfringens (formerly known as C. welchii) and Bacillus cereus.
- ATrue
- BFalse
33why would mycobacterium species that has been stained with spore stain technique show both green and red cells on the slide ?
- Awhen using moist heat some of that myoclic acid melts allowing stain to penetrate . doesant happen for all of the cells which explains the mixture if color
- Bthere must have been a contaminant microorganism present
34what are some ways that contamination can be identified in a culture ?
- Adifferent shape, color
- Btextured colonies
- Camount of growth
35is EMB agar selctive , differential or both ?
- Adifferential lactose fermentation microbes is an ex
- Bselective
- Cnone
- Dboth
36 [blank_start]nutrient Agar[blank_end] is a general purpose medium that will support growth of many common bacteria
- Anutrient Agar
- Benriched media
- Csabouraud agar
37 bacteria growing on or in our bodies often requires [blank_start]enriched media[blank_end] that has been enriched with various special nutrients
- Aenriched media
- Bnutirent agar
- CSAB agar
38The [blank_start]sabouraud[blank_end] agar plate is used to check for fungal spores in the air/nutrient jar was used for bacteria used in surfaces, the enriched agar was used for bacteria on our person.
- Asabouraud- SAB
- BMAC
- Cdeep
39Because microbes are temporary and can be removed by hand washing they are called _____ flora
- Atransient
- Bnormal
- Cresident
40There are species of micro organisms that live naturally and permanently in various areas of our body which are called resident or normal flora
- ATrue
- BFalse
41A nosocomial infection is
- Adefined as a disease acquired during hospitalization.
- Bdefined as a disease acquired after hospitalization
42On most modern microscope are para focal which means
- Athe microscope is binomial
- Bthat when one lenses and focused the others are as well
43A general purpose medium is one that is used merely to grow bacteria and to keep them alive nutrient Agar and nutrient broth are examples
- ATrue
- BFalse
44 An [blank_start]enriched medium[blank_end] contains added nutrients to enhance the growth of those finicky bacteria what the gourmet appetite an example would be the use of blood Agar for growing certain pathogenic microbes
- Aenriched medium
- BSAB agar
- Cnutrient medium
45a [blank_start]selected[blank_end] medium only allows certain species of microorganisms to grow an inhibits others for example EMB contains chemical dyes that inhibit growth of gram positive bacteria and select foreground negative species
- Aselective
- Bdifferential
46a [blank_start]differential[blank_end] medium how's the diagnostic test built into it that changes color with different species of microbes lactose broth will turn from red to yellow for species that can ferment lactose but not for those that can‘t
- Adifferential
- Benriched
- Csimple
47a transport medium is used to transport microbes from one place to another like a doctors office to a diagnostic laboratory the function of a transport medium is to keep the microbes alive without allowing them to overgrow in transfer
- ATrue
- BFalse
48 a _______ culture is defined as a medium growing one an only one intended species of microorganism
- Apure
- Bmixed
- Ccontaminated
49a _____ culture on the other hand contains 2 or more microbes growing together on purpose
- Acontaminated
- Bmixed
- Cpure
50___________ is surface growth
- Apellicle
- Bturbid
- Csediment
51two primary functions for the poor plate is enumeration or counting bacteria // and creating an evenly distributed lawn of microorganisms . the lawns of bacteria are useful for such procedures as phage typing
- ATrue
- BFalse
52the streak plate is performed to accomplish which of the following ?
- Aisolate colonies
- Bdistinguish characteristics
- Cphage typing
- Denumeration
53_____________ is over all shape in this experiment
- Aelevaton
- Bmargin
- Cform
54________-- is profile which means flat, raiased or wrinkled
- Aform
- Bmargin
- Celevaton
55margin is edge which means smooth, filaments , undulate features
- ATrue
- BFalse
56[blank_start]Cocci[blank_end] are spheres and divided into sub categories based on their arrangements.
- ACocci
- Brod
- Cbacilli
57[blank_start]Bacilii[blank_end] are rod shaped and not usually divided by arrangement
- ABacilii
- Bcocci
- Chelix
58[blank_start]Helicoidal[blank_end] are Divided into flexible and rigid types the flexible helicoidal bacteria are called the spirochetes. The rigid ones are divided into spiral shapes and comma types
- AHelicoidal
- Bdiplococci
- Cbacilli
59iodine in gram staining is what ?
- Amordant
- Bdecolorizer
60the decolorizer __________ removes the stain from some bacteria ( gram negative) which becomes colorless but the gram positive does not and remains purple
- Aethanol
- Biodine
61The __________________ proposes that bacteria stain differently because of a chemical and structural difference in the cell wall
- Apermeability theory
- Bscientific method
- Cscientific law
62[blank_start]capsules[blank_end] are significantly medically because they inhibit phagocytosis by our neutrophils and macrophages .pick up is allow certain species of bacteria look capsules to escape our defense and produce infection
- Acapsules
- Bacid stains
- Cnegative staining
63 the capsule stain procedure involves for staining of the background of the slide which is called ______ staining
- Anegative
- Bpositive
- Cacid fast
64capsule staining uses ethanol in its procedure
- ATrue
- BFalse
65which two produce spores?
- Aclostridium
- Bbacillus
- Cecoli
- Dtuberculosis
66Gram stain do not penetrate this spore wall ,this endo spore appear ________- oval shapes
- Ablue/green
- Bunstained / clear
- Cpink
67In todays lab Schaeffer fulton [blank_start]spore stain[blank_end] , hot malachite green penetrates the spore wall to dye spores blue green Any remaining cytoplasm outside the sport is stained red by the counterstain safrinin
- Aspore stain
- Bacid fast
- Cgram stain
68in what staining technique is methylene blue used as a counterstain ?
- Aspore
- Bcapsule
- Cacid fast
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- Quiz on Micro practical questions , created by Katie Gonzalez on 07/03/2019.
- Author
- Katie Gonzalez
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- English
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