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Practical semester 1

Alex Hurtado 16 questions

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Questions in this quiz (16)

  1. 1LS3
    Slope cultures can be made by heating up tubes containing ~10ml agar/gelatin medium. This is done in a sloping position. Why?
    1. AMicrobes spread more easily in a sloped position, as it is harder for bacteriophages to land on sloped surfaces.
    2. BIt's prettier
    3. CTo increase the surface area of the medium.
    4. DTo decrease the volume of the medium.
  2. 2LS3
    Which method of culturing micro-organisms is appropriate for culturing an anaerobic culture of micro-organisms?
    1. ASlope culture
    2. BStab culture
    3. CPlate culture
    4. DPour plate culture
  3. 3LS3
    What makes the use of spread plate culture useful?
    1. AAllows for well spread colonies to be present in the plate.
    2. BAllows for anaerobic cultures to be grown.
    3. CAllows for placement of antibiotics into the culture, for testing antibiotic resistance.
    4. DAllows for applications such as measuring the viable cell count of a bacterial suspension.
  4. 4LS3
    To make a spread plate I counted 10 colonies per plate. The volume of bacterial suspension added was 10ml. The dilution factor of this suspension was 10^4. What was the number of viable bacteria per ml in the undiluted culture?
    1. A906
    2. B1060
    3. C106
    4. D10.6
  5. 5LS3
    When using a Helber slide counting chamber to count algal cells, what organisms should be ignored?
    1. AThe ones on the right and bottom graticule lines.
    2. BThe ones on the left and top graticule lines.
  6. 6LS4
    A bacterial film is made by spreading a loopful of microbial culture onto a clean, grease free slide. How is the newly made bacterial film heat fixed?
    1. ABy holding the slide with tongs with the bacterial film facing down, passing it through a gently burning bunsen flame 3 times.
    2. BBy holding the slide with tongs with the bacterial film facing down, passing it through a gently burning bunsen flame 6 times.
    3. CBy holding the slide with tongs with the bacterial film facing up, passing it through a violently burning bunsen flame 3 times.
    4. DBy holding the slide with tongs with the bacterial film facing up, passing it through a gently burning bunsen flame 3 times.
  7. 7LS4
    What is the difference between a positive stain and a negative stain? Hint: these stains are used as general staining of bacteria.
    1. APositive staining is when the areas outside the organism are coloured. Negative staining is when the areas inside the organism is stained.
    2. BPositive staining is when the areas inside the organism are coloured. Negative staining is when the areas outside the organism is stained.
    3. CPositive staining is when gram positive bacteria are stained and negative staining is when gram negative bacteria are stained.
    4. DPositive staining is when gram negative bacteria are stained and negative staining is when gram positive bacteria are stained.
  8. 8LS4
    What is the difference between gram positive and gram negative bacteria?
    1. AGram positive bacteria have a thick cell wall made of peptidoglycan, which retains methyl violet, whereas Gram negative bacteria have a much thinner cell wall, which does not.
    2. BGram positive bacteria have a thick cell wall made of peptidoglycan, which cannot retain methyl violet, whereas Gram negative bacteria have a much thinner cell wall, which can.
    3. CGram positive bacteria have a thin cell wall made of peptidoglycan, which retains methyl violet, whereas Gram negative bacteria have a much thicker cell wall, which does not.
    4. DGram positive bacteria have a thin cell wall made of peptidoglycan, which cannot retain methyl violet, whereas Gram negative bacteria have a much thicker cell wall, which can.
  9. 9LS4
    Which of the statements below is incorrect?
    1. AGeneral stains act on all bacteria, allowing us to see their general morphology more easily whilst differential stains such as the Gram stain provide us with info about specific structures that make up the bacteria in the sample.
    2. BMicroscope slides can be degreased well enough by chromic acid, which is a stong oxidising agent.
    3. CBacterial films are heat fixed by passing the slide through a gently burning bunsen burner flame three times, with the bacterial film uppermost.
    4. DMethylene blue is a positive general stain, so it colours the organism and the other material fixed on the slide. It is left for 5 minutes and rinsed gently in deionised tap water, to have a clean coverslip placed over the bacteria and observed at 100x with a drop of oil on the coverslip.
  10. 10LS4
    Which of the following statements regarding Gram staining is incorrect?
    1. AGram positive bacteria appear purple-blue
    2. BGram-negative bacteria appear pink red due to the bacteria having only thin peptidoglycan cell walls which do not absorb the violet stain and instead absorb the carbol fuschin counterstain.
    3. CWhen Gram staining occurs, E.coli is rod shaped and chained with purple/violet staining inside and B.megaterium has pink inside with a rod like shape
    4. DOil is used under the 100x objective, not the 40x objective.

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Quiz on Practical semester 1, created by Alex Hurtado on 17/05/2019.
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