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Media for Micro
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Questions in this quiz (97)
1What classification is selenite broth?
- AEnriched
- BSelective
- CDifferential
- DEnriched and Selective
2What specimen(s) is selenite broth effective with isolating?
- ASelective: Isolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
- BSelectively isolates Salmonella species & Yersinia enterocolitica (stool pathogens)
- Cisolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
- DPrimary isolation broth for clinical samples. Used to recover scanty (small or insufficient in quantity or amount) pathogens
- EDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- FUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Gto selectively isolate Vibrio cholera and other Vibrio species from feces
- HUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species.
- ASelective: Isolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
3Which acts as a fermentable sugar and maintains a uniform pH in the selenite broth?
- ALactose
- BSodium Phosphate
- CSodium Selenite
- DPeptone Mixture
4Which substance acts as a buffer in the selenite broth?
- ALactose
- BSodium phosphate
- CSodium selenite
- DPeptone mixture
5Which substance inhibits the growth of Gram positive and many Gram negative bacteria in selenite broth?
- ALactose
- BSodium phosphate
- CSodium selenite
- DPeptone mixture
6Which substance provides a source of nitrogen, vitamins and amino acids for growth of Salmonella in selenite broth?
- ALactose
- BSodium phosphate
- CSodium selenite
- DPeptone mixture
7What temperature and period of time is selenite broth incubated?
- AIt is incubated for 18 hours at 35 degrees Celsius in aerobic conditions. Caps loose.
- BIt is incubated for 24 hours at 37 degrees Celsius in aerobic conditions. Caps loose.
- CIt is incubated for 14 hours at 30 degrees Celsius in aerobic conditions. Caps loose.
- DIt is incubated for 18 hours at 42 degrees Celsius in aerobic conditions. Caps loose.
- AIt is incubated for 18 hours at 35 degrees Celsius in aerobic conditions. Caps loose.
8 Selenite broth should be stored away from direct sunlight
- ATrue
- BFalse
9Which of these are apart of the composition of selenite broth;
- ALactose
- BSodium phosphate
- CSodium selenite
- DPeptone mixture
- EOxbile
- FEsculin
- GFerric Citrate
- HXylose
10After incubation the top portion of the broth is immediately sub-cultured onto Salmonella isolation media-Hektoen or XLD
- ATrue
- BFalse
11What specimen(s) does Bile-Esculin Agar isolate for?
- AIsolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
- BSelectively isolates Salmonella species & Yersinia enterocolitica (stool pathogens)
- Cisolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
- DPrimary isolation broth for clinical samples. Used to recover scanty (small or insufficient in quantity or amount) pathogens
- EDifferential plating medium for the growth and enumeration of urinary tract microorganisms
12What type of classification is this Agar?
- ASelective and differential
- Bselective
- Cdifferential
- Denriched
13What type of incubation is required?
- AAerobic environment at 35 degrees for 24 hours
- Banaerobic environment at 35 degrees for 24 hours
- CAerobic environment at 35 degrees for 48 hours
- Danaerobic environment at 235degrees for 24 hours
14What substance is a selective ingredient that inhibits Gram-positive bacteria other than Enterococci and Group D Streptococci
- AOxbile
- BEsculin
- CFerric Citrate
- DXylose
15Which substance forms a dark brown/black colour on the agar plate after reacting with esculin (on a Bile-Esculin Agar)?
- AOxbile
- BEsculin
- CXylose
- DFerric Citrate
16If Bacteria Hydrolyzes esculin it will react with ferric citrate to produce a black colour, indicating a positive result? (bile-esculin agar)
- ATrue
- BFalse
17It is not Used as an identification test for Group D Streptococci, Enterococci and certain GPBs- Listeria (Bile-esculin agar)
- ATrue
- BFalse
18XLD agar stands for Xylose Deoxycholate agar
- ATrue
- BFalse
19What is Xylose deoxycholate agar applied for?
- Aisolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
- BSelectively isolates Salmonella species & Yersinia enterocolitica (stool pathogens)
- CIsolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
- DPrimary isolation broth for clinical samples. Used to recover scanty (small or insufficient in quantity or amount) pathogens
- EDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- FUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Aisolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
20Is Xylose deoxycholate selective and differential?
- ATrue
- BFalse
21Which substance in XLD agar is fermented by Salmonella and most enteric bacteria but cannot be fermented by Shigella leaving the isolated colonies colourless
- AXylose
- BL-Lysine
- CLactose
- DSodium Thiosulfate & Ferric Ammonia Citrate
- EPhenol Red
- FSodium Deoxycholate
22Which substance in XLD agar leads to decarboxylation by Salmonella after Xylose depleted. Raises pH and thus colonies appears colourless
- ALactose
- BL-Lysine
- CSodium Thiosulfate & Ferric Ammonia Citrate
- DPhenol Red
- ESodium Deoxycholate
23Which pair of substances together are an - indicator of hydrogen sulfide production by Salmonella which results in black dots in the centre of colonies ( In XLD agar)
- AXylose + L-Lysine
- BLactose + Xylose
- CSodium Thiosulfate + Ferric Ammonia Citrate
- DPhenol Red + Ferric Ammonia Citrate
24Phenol red yellow in acidic pH, and red in basic pH; which is an indicator used in XLD agar
- ATrue
- BFalse
25Check all substances found in Xylose deoxycholate that are seen as differential components;
(hint: one component is selective)- AXylose
- BL-Lysine
- CLactose
- DSodium Thiosulfate & Ferric Ammonia Citrate
- EPhenol Red
- FSodium Deoxycholate
26What substance inhibits gram positive bacteria found in Xylose deoxycholate agar?
- AXylose
- BL-Lysine
- CLactose
- DSodium Thiosulfate
- EFerric Ammonia Citrate
- FSodium Deoxycholate
27XLD agar plates are incubated at 35 degrees for 24 hours in aerobic environment
- ATrue
- BFalse
28Is Shigella a NLF making it colourless, Salmonella a NLF with or without H2S - black centers and normal flora LF leading to yellow colonies
- AYes
- BNo
29What does thioglycollate medium with resazurin isloate for?
- APrimary isolation broth for clinical samples. Used to recover scanty (small or insufficient in quantity or amount) pathogens
- BDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- CUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Dto selectively isolate Vibrio cholera and other Vibrio species from feces
- EUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- FIdentification of Pseudomonas aeruginosa
- GSpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
- HThis media is used to isolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- Idetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp.
30What type of classification is thioglycollate medium with resazurin?
- AEnriched
- BDifferential
- CSelective
- DDifferential and Enriched
31How long and what temperature is THIOGLYCOLLATE MEDIUM WITH RESAZURIN incubated for?
- A48-72 hours at 37 degrees
- B48 hours at 42 degrees
- C24 hours at 35 degrees
32What ingredient in THIOGLYCOLLATE MEDIUM WITH RESAZURIN is the reducing agent, it reacts with molecular oxygen keeping free oxygen levels low. In the broth creates a low redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.
- ASodium Thioglycollate
- BResazurin
- CSodium Chloride
- DYeast extract
- ASodium Thioglycollate
33Are Sodium Thioglycollate and L-cysteine reducing agents and are they both found in THIOGLYCOLLATE MEDIUM WITH RESAZURIN?
- ATrue
- BFalse
34Resazurin a redox indicator which is found in THIOGLYCOLLATE MEDIUM WITH RESAZURIN will change from colourless to a certain colour when in an oxidized environment, which colour is it?
- APink
- BYellow
- CGreen
- DRed
35What application is Cysteine Lactose Electrolyte Deficient Agar used for?
- ADifferential plating medium for the growth and enumeration of urinary tract microorganisms
- BUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Cto selectively isolate Vibrio cholera and other Vibrio species from feces
- DUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- EIdentification of Pseudomonas aeruginosa
- FSpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
- GThis media is used to isolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- ADifferential plating medium for the growth and enumeration of urinary tract microorganisms
36Which of these ingredients is the fermentable carbohydrate providing carbon and energy in the Cysteine Lactose Electrolyte Deficient Agar?
- ALactose
- BGlucose
- CSucrose
- DL-cysteine
37Which ingredient is added as a growth supplement for cysteine dependent coliforms in Cysteine Lactose Electrolyte Deficient Agar?
- AL-cysteine
- BLactose
- CBromothymol blue
- DBeef extract
38Which indicator is used in Cysteine Lactose Electrolyte Deficient Agar?
- ABromothymol blue
- BPhenol red
39What temperature, incubation time, and environment is required for a cysteine lactose electrolyte deficient agar?
- A35 + or - 2 degrees for 24 hours in O2
- B42 + or - 2 degrees for 48 hours in O2
- C35 + or - 2 degrees for 48 hours in CO2
- D35 + or - 2 degrees for 72 hours in O2
40What application/purpose are DNase agar plates used for?
- AUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Bto selectively isolate Vibrio cholera and other Vibrio species from feces
- CUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- DIdentification of Pseudomonas aeruginosa
41DNase agar plate is differential
- ATrue
- BFalse
42DNase agar is used to test whether an organism is able to hydrolyze DNA
- ATrue
- BFalse
43Which two of these are metachromatic dyes which indicate a colour change and presence of the enzyme DNase? This is also a selective ingredient used in the DNase test agar.
- AToluidine Blue
- BMethyl Green
- CBromothymol Blue
- DPhenol Red
44If the medium does not contain a metachromatic dye then what do we inoculate after incubation? Also what is a negative reaction and what is a positive reactions characteristics? On a DNase agar plate
- A1 N HCl, precipitate with cloudiness for negative and clearing around the organism for positive
- B1 N HCl, precipitate with cloudiness for positive and clearing around the organism for negative
- C2 N HCl, precipitate with cloudiness for positive and clearing around the organism for negative
- D2 N HCl, precipitate with cloudiness for negative and clearing around the organism for positive
45What does the abbreviation "TCBS" in TCBS agar stand for
- AThiosulphate Citrate Bile Salts Sucrose
- BThiosulphate Citrate Bile Sucrose
- CThiosulphate Citrate Bile Salts
- DThiosulphate Cysteine Bile Salts
46What is the purpose of Thiosulphate Citrate Bile Salts Sucruse agar in terms of isolation of organism(s)?
- Ato selectively isolate Vibrio cholera and other Vibrio species from feces
- BUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- CIdentification of Pseudomonas aeruginosa
- DSpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
- EThis media is used to isolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- FSelectively isolates Salmonella species & Yersinia enterocolitica (stool pathogens)
- GIsolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
- Ato selectively isolate Vibrio cholera and other Vibrio species from feces
47What type of classification is thiosulphate citrate bile salts sucrose agar?
- ASelective
- Bdifferential
- Cenriched
- Dselective and differential
48Which of these ingredients in Thiosulphate citrate bile salts sucrose agar provide the nutrients, vitamins, amino acids, and nitrogen Vibro spp. require for growth?
- AYeast extract
- BEnzymatic digest of animal tissue
- CEnzymatic digest of casein
- DSodium citrate
- ESodium thiosulphate
- AYeast extract
49Which of these ingredients in Thiosulphate citrate bile salts sucrose agar make the media selective: by providing a basic/alkaline pH, Inhibit the growth of gram positive organisms and suppress coliforms. Pick all that apply
- ASodium cholate
- BSodium citrate
- CSodium thiosulphate
- DOxbile
- EBile salts
- FYeast extract
- GEnzymatic digest of animal tissue
- HEnzymatic digest of casein
- ASodium cholate
50Sucrose used in TCBS agar allows for the differentiation of Vibrio sp. that ferment sucrose
- ATrue
- BFalse
51Sodium Chloride used in TCBS helps maintain osmotic balance
- ATrue
- BFalse
52Ferric Citrate and Sodium thiosulphate in the TCBS agar act together to detect the production of hydrogen sulfate (H2S) which is shown by the production of a black precipitate
- ATrue
- BFalse
53Bromthymol blue and Thymol blue are not used as pH indicators in the TCBS agar
- ATrue
- BFalse
54What is the purpose for using Löwenstein–Jensen?
- AUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- Bisolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
- CPrimary isolation broth for clinical samples. Used to recover scanty (small or insufficient in quantity or amount) pathogens
- DDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- EUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- Fto selectively isolate Vibrio cholera and other Vibrio species from feces
- Gdetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp
- HUsed to isolate stool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni
55What classification is given to the Löwenstein–Jensen medium?
- ASelective and Enriched
- BDifferential
- CSelective
- DSelective and Differential
56What incubation requirements are required for a Löwenstein–Jensen medium
- AAmbient air with 5-10% CO2 for 6-9 weeks in the dark 33-37C
- BAmbient air with 5-10% CO2 for 5 weeks in the dark 33-37C
- CAmbient air with 5-10% CO2 for 6-9 weeks in the light 33-37C
- DAmbient air with 5-10% O2 for 6-9 weeks in the dark 33-37C
57Which ingredients of the Löwenstein–Jensen medium Inhibits growth of gram positive and gram negative bacteria?
- APenicillin and Eggs
- BPenicillin and Nalidixic acid
- CEggs and Malachite green
- DMalachite green and Penicillin
58This media is used as the primary isolation media to determine the presence of Mycobacterium tuberculosis
- ATrue
- BFalse
59M. tuberculosis is highly infectious and a potential airborne infection. Because of this it must be grown in a capped tube
- ATrue
- BFalse
60What organism(s) is Cetrimide Agar used for?
- AIdentification of Pseudomonas aeruginosa
- BSpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
- C isolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- Ddetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp.
- Eisolate stool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni.
- FUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- Gto selectively isolate Vibrio cholera and other Vibrio species from feces
61Which ingredient in cetrimide agar is an enzyme that provides nutrients necessary for bacterial growth?
- APancreatic Digest
- BMagnesium Chloride
- CPotassium Sulfate
- DAmmonium Compound
62Which two ingredients in cetrimide agar stimulate the production of two pigments pyoverdin (yellow) and pyocyanin (blue)
- AMagnesium Chloride and Potassium Sulphate
- BMagnesium Chloride and Ammonium Compound
- CCetrimide and Pancreatic Digest of Gelatin
- DPotassium Sulphate and Cetrimide
63Which ingredient in Cetrimide agar is Inhibitory to a wide variety of bacterial species including Pseudomonas, excluding P. aeruginosa?
- ACetrimide
- BPotassium Sulphate
- CMagnesium Chloride
- DPancreatic Digest of Gelatin
64OFTEN TIMES, THIS CETRIMIDE AGAR IS SUPPLEMENTED WITH 1% GLYCEROL AS A SOURCE OF CARBON.
- ATrue
- BFalse
65What is the required incubation environment for Cetrimide Agar
- A35-37°C , 18 to 24 hours, O2
- B35-37°C , 48 to 72 hours, O2
- C35-37°C , 18 to 24 hours, CO2
- D42°C , 18 to 24 hours, O2
- A35-37°C , 18 to 24 hours, O2
66Yellow-green colour and smell of grapes is not indicative of P. aeruginosa
- ATrue
- BFalse
67Cetrimide Agar is widely recommended for use in the examination of cosmetics, clinical specimens for the presence of P. aeruginosa, as well as for evaluating the efficacy of disinfectants against this organism. It is also used in the microbiological examination of nonsterile pharmaceutical products for pseudomonas aeruginosa.
- ATrue
- BFalse
68P. aeruginosa is found virtually everywhere!
Environmental bacteria found in soil, water, even surfaces of plants and animals and also an opportunistic Pathogen- ATrue
- BFalse
69P. aeruginosa ARE NOT PRIMARILY FOUND AS NOSOCOMIAL INFECTIONS
(Cetrimide agar)- ATrue
- BFalse
70What is the purpose of the Amies transport media?
- ASpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
- BIsolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- CDetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp
- DUsed to isolate stool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni.
- EIdentification of Pseudomonas aeruginosa
- FUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- Gto selectively isolate Vibrio cholera and other Vibrio species from feces
- HUsed in detecting the enzyme deoxyribonuclease to identify Staphylococcus aureus, Moraxella, Serratia
- IDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- JIsolate and differentiate gram negative enteric pathogens: Salmonella and Shigella
- ASpecimen collection, preservation (prolong microorganisms’ survival) and transportation to the laboratory
71With Charcoal used as an ingredient for the amies transport medium, what transporting environment should be maintained?
- ApH at 25 °C : 7.2 ± 0.2 in O2
- BpH at 25 °C : 7.3 ± 0.2 in O2
72Without Charcoal used as an ingredient for the amies transport medium, what transporting environment should be maintained?
- ApH at 25 °C : 7.2 ± 0.2 with O2
- BpH at 25 °C : 7.3 ± 0.2 with O2
- ApH at 25 °C : 7.2 ± 0.2 with O2
73Sodium, Calcium, Magnesium and potassium salts in the amies transport medium help by; Pick all that apply
- Acontrolling the permeability of bacterial cells
- Bhelps maintain bacterial cell survival
- Cprovide electrolytes required for transport and maintaining osmotic balance
74Charcoal in media contributes to
1) neutralize toxins, fatty acids, and inhibitory material.
2)promote growth of fastidious pathogens such as Neisseria gonorrhoeae- ATrue
- BFalse
75What is the Martin Lewis Agar used to isolate for?
- AIsolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- BDetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp.
- CUsed to isolate stool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni.
- DIdentification of Pseudomonas aeruginosa
- EThe cultivation of Mycobacterium tuberculosis and other Mycobacterium species.
- FTo selectively isolate Vibrio cholera and other Vibrio species from feces
- GDifferential plating medium for the growth and enumeration of urinary tract microorganisms
- HPrimary isolate media for clinical samples (stool) to isolate Shigella and Salmonella. Inhibits normal flora
76What classification is given to the Martin Lewis Agar?
- ASelective and Enriched
- BSelective
- CEnriched
- DDifferential and Selective
77The ingredient in the Martin Lewis Agar that inhibits Gram-negative bacteria is?
- AColistin
- BVancomycin
- CAnisomycin
- DTrimethoprim
78The ingredient in the Martin Lewis Agar that inhibits Gram-positive bacteria is?
- AColistin
- BVancomycin
- CAnisomycin
- DTrimethoprim
79Which ingredient that is found in the Martin Lewis Agar that inhibits the growth of yeasts is?
- AColistin
- BVancomycin
- CAnisomycin
- DTrimethoprim
80Which ingredient in the Martin Lewis agar inhibits the Proteus spp.
- ATrimethoprim
- BAnisomycin
- CVancomycin
- DColistin
81Inoculation of the Martin Lewis Agar plate is done first, then streak for isolation, and incubate at 35-37ºC in an atmosphere enriched with CO2 for 18-72 hours
- ATrue
- BFalse
82 Martin Lewis agar also known as a chocolate agar
- ATrue
- BFalse
83What does the abbreviation "PEA" agar with blood stand for?
- APhenylethyl Alcohol
- BPhosphatethyl Acomyosin
- CPotassiumethyl Ammonium
84What type of Agar is PEA agar classified as?
- AEnriched and Selective
- BEnriched
- CDifferential and Selective
- DDifferential and Enriched
85What does blood phenylethyl alcohol agar detect and isolate for?
- AGram positive cocci such as Staphylococci and Streptococci spp.
- Bstool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni
- Cpathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- DSalmonella species & Yersinia enterocolitica (stool pathogens)
- EIsolates Group D Streptococci and Enterococci (main purpose) live in our gut these organisms
- Fgram negative enteric pathogens: Salmonella and Shigella
- AGram positive cocci such as Staphylococci and Streptococci spp.
86Incorporation of phenylethyl alcohol, which reversibly inhibits DNA synthesis and thus inhibits facultative anaerobic gram negative bacteria such as the members of the family Enterobacteriaceae. PEA inhibits swarming Proteus spp, Would this make it selective or enriched
- ASelective
- BEnriched
87For PEA agar, 5% sheep blood supports growth of gram positives. Would this be classified as enriched or selective?
- AEnriched
- BSelective
88For phenylethyl alcohol agar with 5% sheep blood, which two ingredients present provide additional nutrient such as carbon, nitrogen, sulfur and trace elements to support growth of organism
- APapaic Digest of Soybean Meal and Pancreatic Digest of Casein
- BPapaic Digest of Soybean Meal and Sodium Chloride
- Cß-Phenylethyl Alcohol and Pancreatic Digest of Casein
- Dß-Phenylethyl Alcohol and Sodium Chloride
89Which ingredient in PEA agar maintains osmotic equilibrium?
- ASodium Chloride
- Bß-Phenylethyl Alcohol
- C5% Sheep Blood
- DPapaic Digest of Soybean Meal
- EPancreatic Digest of Casein
90Which ingredient in PEA agar inhibits DNA synthesis of facultative anaerobic gram negative bacteria?
- Aß-Phenylethyl Alcohol
- BSodium Chloride
- CPapaic Digest of Soybean Meal
- DPancreatic Digest of Casein
- E5% Sheep Blood
91PEA agar is used to inhibit facultative gram-negative rods, preventing Enterobacteriaceae from overgrowing the anaerobes, and inhibiting swarming of Proteus spp. and Clostridium septicum
- ATrue
- BFalse
92PEA agar is not used for purulent specimens and when mixed infections are suspected, and PEA agar with 5% sheep blood is not used to isolate most gram-positive and gram-negative anaerobes from clinical samples
- ATrue
- BFalse
93What is the purpose of Cary-Blair medium?
- AUsed for collection and transportation of stool and rectal swabs to isolate stool pathogens- gram negative bacilli, such as Salmonella, Shigella, and Campylobacter jejuni
- Bdetection and isolation of: Gram positive cocci such as Staphylococci and Streptococci spp.
- Cisolate pathogenic Neisseria sp. (Neisseria gonorrheae, Neisseria meningitidis) from specimens containing mixed flora
- DIdentification of Pseudomonas aeruginosa
- EUsed for the cultivation of Mycobacterium tuberculosis and other Mycobacterium species
- Fto selectively isolate Vibrio cholera and other Vibrio species from feces
94Which ingredient in the cary-blair transport medium acts as a buffering agent to prevent overgrowth of enteric pathogens and normal flora
- ADisodium hydrogen phosphate
- BSodium thioglycollate
- CSodium chloride and calcium chloride
- DpH of 8.4
95Which ingredient in the cary-blair transport mediums purpose is to impede oxidation, which allows bacteria to survive for longer periods on the media
- ADisodium hydrogen phosphate
- BSodium thioglycollate
- CSodium chloride and calcium chloride
- DpH of 8.4
96Which two ingredients in the cary-blair medium is used to maintain the osmotic equilibrium, which aids in the preservation of the bacterial cells?
- ADisodium hydrogen phosphate and Sodium thioglycollate
- BSodium chloride and calcium chloride
- CSodium thioglycollate and pH of 8.4
- DpH of 8.4 and Disodium hydrogen phosphate
97pH of 8.4 for the cary-blair medium minimizes the destruction of bacteria due to acid formation as the sample is being transported
- ATrue
- BFalse
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- Quiz on Media for Micro, created by persha.hossaini on 24/01/2015.
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- persha.hossaini
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