Quiz
MicroBio 2440 Lab midterm
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Questions in this quiz (42)
1Match the waste items below with the appropriate receptacle.
Glass slides [blank_start]sharps container[blank_end]
Used test tubes [blank_start]racks in back by sink[blank_end]
used agar plates [blank_start]biohazard bin[blank_end]
stain runoff in tray [blank_start]chemical waste carboy[blank_end]
paper towel soaked in ethanol [blank_start]regular trash[blank_end]- Asharps container
- Bregular trash
- Cbio hazard bin
- Dracks in the back by sink
- Echemical waste carboy
- Fracks in back by sink
- Gsharps container
- Hregular trash
- Ibio hazard bin
- Jchemical waste carboy
- Kbiohazard bin
- Lsharps container
- Mregular trash
- Nracks in the back by sink
- Ochemical waste carboy
- Pchemical waste carboy
- Qsharps container
- Rregular trash
- Sbiohazard bin
- Tracks in the back by sink
- Uregular trash
- Vsharps container
- Wbiohazard bin
- Xcks in the back by sink
- Ychemical waste carboy
2Our lab is a BSL-[blank_start]2[blank_end] lab. This means that the organisms used in this lab are [blank_start]commonly[blank_end]
encountered in the community and present a [blank_start]moderate[blank_end] environmental and/or health hazard.
Although they are associated with a number of human diseases, these diseases can be
[blank_start]treated[blank_end] if identified in a timely manner.- A2
- Bcommonly
- Cmoderate
- Dtreated
3What are some safety precautions one must take in a BSL-2 lab?
proper [blank_start]attire[blank_end], [blank_start]gloves[blank_end], [blank_start]autoclave[blank_end] for waste disposal, [blank_start]biohazard[blank_end] bins, [blank_start]sharps[blank_end] container- Aattire
- Bgloves
- Cautoclave
- Dbiohazard
- Esharps
4What are some pieces of personal protective equipment we use in our BSL-2 lab?
[blank_start]gloves[blank_end], [blank_start]closed[blank_end]-[blank_start]toed[blank_end] shoes, [blank_start]eye[blank_end] protection, etc- Agloves
- Bclosed
- Ctoed
- Deye
5Before you begin working, you should always:
Wash your [blank_start]____________[blank_end]
Wipe down your workbench with [blank_start]_______________[blank_end]
Put on appropriately sized [blank_start]_____________[blank_end]
Whenever you are finished working, you should always:
Throw away waste in the appropriate [blank_start]_________________[blank_end]
Wipe down your workbench with [blank_start]______________[blank_end]
Wash your [blank_start]___________[blank_end]- Ahands
- Bethanol
- Cgloves
- Dwaste containers
- Eethanol
- Fhands
6We flame the loop before and after using it to prevent contamination.
- ATrue
- BFalse
7Eating and drinking: [blank_start]yes no[blank_end]
Texting: [blank_start]yes no[blank_end]
Applying makeup: [blank_start]yes no[blank_end]
Applying lip balm: [blank_start]yes no[blank_end]
Coming in outside of lab times: [blank_start]yes no[blank_end]
Coming in during other labs: [blank_start]yes no[blank_end]
Run around the room: [blank_start]yes no[blank_end]
Take pictures with your phone: [blank_start]yes no[blank_end]- Ayes
- Bno
- Cyes
- Dno
- Eyes
- Fno
- Gyes
- Hno
- Iyes
- Jno
- Kyes
- Lno
- Myes
- Nno
- Oyes
- Pno
8What conditions are used in an autoclave cycle?
Temp: [blank_start]121 C[blank_end]
psi: [blank_start]15 psi[blank_end]
time: [blank_start]15 minutes[blank_end]- A121 C
- B15 psi
- C15 minutes
9[blank_start]Fomite[blank_end] - object likely to be a mode of transportation for pathogenic microbes
- AFomite
10[blank_start]colony forming units[blank_end]: unit used to estimate the number of visible cells in a sample
- Acolony forming units
11[blank_start]mixed culture[blank_end]: 2 or more species of bacteria within a culture
- Amixed culture
12[blank_start]turbidity[blank_end]: measure of cell density in a broth culture (degree of [blank_start]cloudiness[blank_end])
- Aturbidity
- Bcloudiness
13[blank_start]aseptic[blank_end]: free from contamination caused by pathogenic microbes
- Aaseptic
14[blank_start]defined media[blank_end]: all ingredients known- concentration and amount
- Adefined media
15defined media includes animal and biological products
- ATrue
- BFalse
16[blank_start]complex media[blank_end]: contains material of biological origin so can't be defined
- Acomplex media
17In a streak plate for isolation, it does matter in which quadrant isolated colonies appear.
- ATrue
- BFalse
18What is the goal of a streak plate for isolation?
To obtain [blank_start]isolated[blank_end] colonies of microbes for [blank_start]morphological[blank_end] study. Separates organisms in a [blank_start]mixed[blank_end] culture.- Aisolated
- Bmorphological
- Cmixed
194. Why are the two incubators we commonly use set at 25ºC and 37ºC?
25ºC [blank_start]room temp[blank_end]
37ºC [blank_start]body temp[blank_end]- Aroom temp
- Bbody temp
- Cbody temp
- Droom temp
20state of being everywhere
- Aubiquity
- Bturbidity
- Caseptic
21What practical consequences does microbial ubiquity have?
[blank_start]Healthcare[blank_end]- aseptic technique is critical to preventing spread of [blank_start]pathogens[blank_end] to patients
[blank_start]Lab setting[blank_end]- [blank_start]contamination[blank_end] of cultures and experiments- AHealthcare
- Bpathogens
- CLab setting
- Dcontamination
22label
- Afine focus
- Bstage
- Cdiaphragm
- Dbase
- Elight source
- Fcondenser
- Gstage clip
- Hobjective lenses
- Iocular tube
- Jcoarse focus
23 What is involved in a negative stain?
Used for bacteria that cannot withstand [blank_start]heat[blank_end] fixing.
use an [blank_start]acidic[blank_end] stain ([blank_start]Nigrosin[blank_end]) to stain [blank_start]background[blank_end] of slide- Aheat
- Bacidic
- CNigrosin
- Dbackground
24What is involved in a capsule stain?
Use [blank_start]acidic[blank_end] and [blank_start]basic[blank_end] stains. [blank_start]Acidic[blank_end] stain background so you can see the capsule. [blank_start]Basic[blank_end] stains the cell within the capsule.- Aacidic
- Bbasic
- CAcidic
- DBasic
25Make a smear on a clean microscope slide. Allow the smear to
[blank_start]_____________[blank_end]. When the smear is dry, [blank_start]______________[blank_end] the smear.
Apply [blank_start]_________________[blank_end] for [blank_start]_________[blank_end] second(s).
Apply [blank_start]_________________[blank_end] for [blank_start]_________[blank_end] second(s).
Decolourise with [blank_start]_______________[blank_end] until [blank_start]______________________[blank_end].
Apply [blank_start]_________________[blank_end] for [blank_start]_________[blank_end] second- Aair dry
- Bheat fix
- Ccrystal violet
- D60
- Eiodine
- F60
- Gacid alcohol
- Hclear run off
- Isafranin
- J60
26What is the total magnification of the oil immersion lens when you factor in the eyepiece (10X)?
- A1000
- B10,000
- C100
27When can you use the coarse focus knob? The fine focus?
FF- [blank_start]any[blank_end] objective. [blank_start]refining[blank_end].
CF- [blank_start]initial[blank_end] focusing. [blank_start]4x[blank_end] lens.- Aany
- Brefining
- Cinitial
- D4x
28What are the purposes of each of these components that make up a stain?
[blank_start]Solvent[blank_end]: carries chromogen and aids in permeability
[blank_start]Chromophore[blank_end]: colored part of chromogen
[blank_start]Auxochrome[blank_end]: charged part of chromogen- Asolvent
- Bchromogen
- Cauxochrome
- Dchromophore
- Eauxochrome
- Fsolvent
- Gauxochrome
- Hchromophore
- Isolvent
29Switch on the microscope and start on the [blank_start]_______[blank_end] objective lens. Focus the microscope using the [blank_start]_______________________________[blank_end] and visualize the stain.
Move to the [blank_start]__________[blank_end], then the [blank_start]___________[blank_end], using the [blank_start]___________________________[blank_end]_to refocus the lens.
When using the 100X lens, it is imperative to use
[blank_start]__________________________[blank_end]. When finished, move to the [blank_start]________[blank_end] objective lens. Wipe the [blank_start]________________[blank_end] off the [blank_start]100X[blank_end] lens using [blank_start]_________[blank_end]. Switch off the microscope and wrap the [blank_start]_________________[blank_end] neatly around the base- A4x
- Bcoarse focus
- C10x
- D40x
- Efine focus
- Flens oil
- G4x
- Hlens oil
- I100X
- Jlens paper
- Kcord
30What is the difference between a Gram-negative cell and a Gram-positive cell?
GN- [blank_start]pink[blank_end] because of [blank_start]safranin[blank_end]. Outer membrane [blank_start]present[blank_end]. [blank_start]Thin[blank_end] peptidoglycan layer.
GP- [blank_start]purple[blank_end] because of crystal violet. [blank_start]No[blank_end] outer membrane. [blank_start]Thick[blank_end] peptidoglycan layer.- Apink
- Bsafranin
- Cpresent
- DThin
- Epurple
- FNo
- GThick
31What makes a stain basic or acidic?
Charge of [blank_start]auxochrome[blank_end].
Basic= [blank_start]positive[blank_end]
Acidic= [blank_start]negative[blank_end]
[blank_start]Basic[blank_end] is attracted to cell, [blank_start]acidic[blank_end] is repelled- Aauxochrome
- Bpositive
- Cnegative
- DBasic
- Eacidic
32How does a bacterial capsule enhance virulence?
protection from [blank_start]phagocytosis[blank_end]
protection from loss of [blank_start]moisture[blank_end]
mediates adherence of certain [blank_start]molecules[blank_end] to surface- Aphagocytosis
- Bmoisture
- Cmolecules
33The capsule is not stained in a capsule stain because it has no charge.
- ATrue
- BFalse
34What happens to a bacterial cell in a hypotonic solution? In a hypertonic solution?
[blank_start]Hypotonic[blank_end] = lyse bc water flows in
[blank_start]Hypertonic[blank_end]= plasmolysis bc water leaves- AHypotonic
- BHypertonic
- CHypertonic
- DHypotonic
35[blank_start]Tonicity[blank_end]- measure of osmotic pressure (hyper/hypo/iso)
[blank_start]Osmotic pressure[blank_end]- the pressure of solution against a semipermeable membrane to prevent water flowing inward- ATonicity
- BOsmotic pressure
36What effect does UV-C light have on bacterial cells?
Damages [blank_start]DNA[blank_end] by creating [blank_start]thymine[blank_end] dimers- ADNA
- Bthymine
37Pathogen: [blank_start]disease-causing microbe[blank_end]
Ubiquity: [blank_start]state of being everywhere[blank_end]
thermophile: [blank_start]>40C[blank_end]
Psychrophile: [blank_start]<20C[blank_end]
Psychrotroph: [blank_start]able to tolerate temp as low as 0C[blank_end], but the optimal temp is 20-40C
Mesophile: [blank_start]15-40C. found in the human body[blank_end]
Acidophile: [blank_start]pH 5.5 of less[blank_end]
Alkaliphile: [blank_start]pH 8.5 or more[blank_end]
Neutrophile: [blank_start]pH range of 5.5-8.5[blank_end]
Halophile: [blank_start]NaCl concentration greater than 3%[blank_end]
Plasmolysis: [blank_start]contents of cell pull away[blank_end] from membrane due to loss of water
Osomotolerant: grow at a wide range of NaCl- Adisease-causing microbe
- Bstate of being everywhere
- C<20C
- DpH range of 5.5-8.5
- Estate of being everywhere
- FpH range of 5.5-8.5
- GpH 8.5 or more
- H>40C
- I<20C
- JpH 8.5 or more
- K5-40C. found in the human body
- L<20C
- M5-40C. found in the human body
- NNaCl concentration greater than 3%
- Oable to tolerate temp as low as 0C
- Pstate of being everywhere
- Q>40C
- R15-40C. found in the human body
- SpH 5.5 of less
- TpH range of 5.5-8.5
- U>40C
- VpH 5.5 of less
- Wdisease-causing microbe
- X5-40C. found in the human body
- YpH 8.5 or more
- ZpH 8.5 or more
- AA>40C
- AB<20C
- ACpH 5.5 of less
- ADpH range of 5.5-8.5
- AEpH 5.5 of less
- AF>40C
- AGNaCl concentration greater than 3%
- AHgrow at a wide range of NaCl
- AIcontents of cell pull away from membrane
- AJcontents of cell pull away
- AKcontents of cell pull toward
38Cardinal temperatures: min, max, optimum temp an organism survives at
- ATrue
- BFalse
39Heat control incubation: [blank_start]cardinal[blank_end] temps
Extreme freezing temps: cell structures are [blank_start]damaged[blank_end] from ice crystals- Acardinal
- Bdamaged
40What is the difference between planktonic growth and biofilm growth?
planktonic growth= [blank_start]free floating[blank_end]
biofilm growth= [blank_start]adhere to surface, quorum sensing,[blank_end] stationary and in a community- Afree floating
- Badhere to surface, quorum sensing, stati
- Cadhere to surface, quorum sensing,
- Dfree floating
41Define each of the stages of biofilm growth:
[blank_start]Reversible attachment[blank_end]: planktonic/free floating. attach and detach freely
[blank_start]Irreversible attachment[blank_end]: cell adhesion structures formed, biofilm begins to grow
[blank_start]Growth[blank_end]: growth of biofilm in 2D; quorum sensing begins
[blank_start]Maturation[blank_end]: growth of biofilm in 3D; extracellular matrix strengthed
[blank_start]Dispersal[blank_end]: planktonic cells break off and leave to create another biofilm- AReversible attachment
- BIrreversible attachment
- CGrowth
- DMaturation
- EDispersal
- FIrreversible attachment
- GReversible attachment
- HGrowth
- IMaturation
- JDispersal
- KGrowth
- LReversible attachment
- MIrreversible attachment
- NMaturation
- ODispersal
- PMaturation
- QDispersal
- RIrreversible attachment
- SReversible attachment:
- TGrowth
- UDispersal
- VIrreversible attachment:
- WReversible attachment:
- XGrowth
- YMaturation
42What are the cardinal temperatures for bacteria?
ALL bacteria grow between the range of [blank_start]10C[blank_end] and [blank_start]110C[blank_end]- A10C
- B110C
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- Quiz on MicroBio 2440 Lab midterm, created by Elissa Atkinson on 20/02/2024.
- Author
- Elissa Atkinson
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