Genetics

Description

DNA Polymorphisms and Human Identification MB ASCP
mona1nyc
Quiz by mona1nyc, updated more than 1 year ago
mona1nyc
Created by mona1nyc over 8 years ago
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Resource summary

Question 1

Question
Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?
Answer
  • Restriction fragment length polymorphisms
  • Short tandem repeats
  • Variable number tandem repeats
  • Single nucleotide polymorphisms
  • Chromosomal translocation

Question 2

Question
Which of the following polymorphisms can be detected through Southern blot?
Answer
  • Short tandem repeats
  • Single nucleotide polymorphisms
  • Restriction fragment length polymorphisms
  • Long interspersed nucleotide sequences

Question 3

Question
What does it mean, that a locus is homozygous?
Answer
  • many different alleles
  • two alleles that are the same
  • two alleles that are different
  • no alleles
  • one allele have been deleted

Question 4

Question
The following is a description of which method. Patients DNA was isolated and cut with HaeIII. Product of the restriction digest was resolved by gel electrophoresis and transferred to a membrane. Then the membrane was exposed to hybridization probes, and the resulting band pattern was compared with that seen in another sample of DNA.
Answer
  • RFLP by Southern blotting
  • VNTR by PCR
  • SNP by sequencing
  • STR by PCR
  • SNP by Western Blot

Question 5

Question
FGA, D8S1179, and D2S11 are examples of which of the following?
Answer
  • RFLP
  • VNTR
  • STR
  • SNP

Question 6

Question
Which of the following is used as a marker of gender?
Answer
  • D8S1179
  • Amelogenin
  • TPOX
  • PentaD
  • CD4

Question 7

Question
How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?
Answer
  • 10
  • 12
  • 13
  • 16
  • 20

Question 8

Question
When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?
Answer
  • 1
  • 2
  • 4
  • 6
  • 25

Question 9

Question
Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?
Answer
  • STR
  • Y-STR
  • RFLP
  • VNTR
  • SNP

Question 10

Question
Which of the following STRs is a Y-STR?
Answer
  • D5S818
  • vWA
  • Penta E
  • DYS385
  • LPL

Question 11

Question
A child has disputed paternity. The child has the following STR haplotype: TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28. Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33. What can be said of Alleged Father 1?
Answer
  • He is the father of the child
  • He cannot be excluded as the father
  • He is excluded as the father.
  • More STRs need to be analyzed before a conclusion can be made.

Question 12

Question
An individual who has two populations of cells that were derived from two different zygotes is called a:
Answer
  • chimera
  • mosaic
  • heteroplasmy
  • mutant

Question 13

Question
A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?
Answer
  • RFLP
  • STR
  • Y-STR
  • VNTR

Question 14

Question
DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?
Answer
  • Female
  • Male
  • Impossible to tell from one locus
  • This locus does not discriminate between males and females

Question 15

Question
STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?
Answer
  • To determine whether the donor and recipient are a match
  • To determine the likelihood of graft-versus-host disease
  • To find informative alleles for post-transplantation studies
  • To add their STR haplotypes to a national database

Question 16

Question
STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?
Answer
  • vWA and TH01
  • TH01 and D13S317
  • D16S539 and F13A01
  • vWA and D13S317

Question 17

Question
Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?
Answer
  • 25%
  • 34%
  • 66%
  • 75%

Question 18

Question
Two people have what percentage of DNA sequence that differs between them?
Answer
  • 1%
  • 5%
  • 10%
  • 25%
  • 15%

Question 19

Question
Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?
Answer
  • Y-STR
  • Mitochondrial DNA
  • RFLP-PCR
  • SNP haplotypes

Question 20

Question
Consider the following STR analysis: Which of the child alleles are inherited form the father?
Answer
  • D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 9
  • D3S1358: 4, vWA: 5, FGA: 20, TH01: 11 , TPOX: 10, CSF1PO: 13, D5S818: 3, D13S317: 6
  • D3S1358: 15, vWA: 17, FGA: 23, TH01: 6 , TPOX: 11, CSF1PO: 12, D5S818: 10, D13S317: 10
  • D3S1358: 10, vWA: 17, FGA: 24, TH01: 6 , TPOX: 10, CSF1PO: 12, D5S818: 10, D13S317: 9
  • D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 10

Question 21

Question
Basked on the results which of the alleged fathers (AF) is the father?
Answer
  • AF1
  • AF2
  • Neither

Question 22

Question
A child and an alleged father (AF) share alleles with the following paternity index: What is the combined paternity index from these three loci?
Answer
  • 4.131
  • 4.5
  • 5
  • 3.131

Question 23

Question
With 50% prior odds, what is the probability of paternity from these three loci?
Answer
  • 80%
  • 76%
  • 90%
  • 60%

Question 24

Question
What is a traditional definition of a polymorphism?
Answer
  • The genets variation must be present in at least 1 % of the population.
  • The genets variation must be present in at least 5 % of the population.
  • The genets variation that no longer is present in the population.
  • The genets variation can not be present in majority of the population.

Question 25

Question
Which of the following genomic variations is the hardest to detect?
Answer
  • SNP
  • STRs
  • VNTRs
  • RFLPs

Question 26

Question
When comparing a generic similarity go two random individuals, what percentage of their genome is identical?
Answer
  • 99.5%
  • 95%
  • 90%
  • 100%
  • 80%

Question 27

Question
RFLP stands for?
Answer
  • Restriction fragment length polymorphism
  • Rapidly Fusing length polymorphisms
  • Rescue factor logical polymorphisms
  • Redundant fragment length polymorphism

Question 28

Question
Closely linked markers are used for:
Answer
  • Accurate mapping of regions associated with phenotypic characteristics
  • Finding the length of a sequence
  • Location of the centromere
  • Defining the promoter
  • Disabling the binding of transcription factors

Question 29

Question
What is a definition of a haplotype?
Answer
  • A block of closely linked SPS on the same chromosome that tend to be inherited together.
  • A half of a chromosome.
  • Chromosome with only one functioning allele.
  • Allele that gives a disfiguring phenotype.

Question 30

Question
Mitochondrial DNA is____
Answer
  • circular double stranded
  • circular single stranded
  • linear double stranded
  • linear single stranded

Question 31

Question
Genes encoded on the mtDNA include:
Answer
  • 22 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
  • 30 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
  • 22 tRNA genes, 6 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
  • 22 tRNA genes, 2 ribosomal RNA genes, and 60 genes coding for components of the oxidation-phosphorylation system.

Question 32

Question
Mitochondrial DNA follow which type of inheritance?
Answer
  • Maternal
  • Paternal
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