Electrophoresis

Electrophoresis

1. intro

   • based on differential rate of migration in applied dc
   • inside (silanol gps deprotanated )at pH>3
   • silanol+ cations= rigid layer immobile
   • EOf vs Electrophoretic mobility

2. Detection (cathode driven by EOF)

   • polyamide (part) removed from capillary
   • path length =50-100 μm
   • UV is used ,on column impairs sensitivity short path
     • Z cell
     • Multiple reflections to reduce path
   • Fluorescence / mass spectro (high price)

3. 4 Modes

4. Vs HPLC

   • electrosmotic (no pressure drop ;charge , uniform) vs hydrodynamic flow
   • van deemeter X A , c only B
   • resolution , small sample size, low waste, speed
   • Dis: irrproducible, no scale up, analyte insolubility in BGE

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