Quantal Analysis

Is it a Pre-S change?
1. Coefficent of Variance
  1. If low P(r) then variance about the mean will be high - more scope for fluctuations
    1. Therefore a decrease in the CV indicates an increase in P(r)
  2. No change in CV = no change in P(r) = post S mech
  3. But SILENT SYNAPSES - if LTP un-silences a synapse, then CV will increase with LTP, even though no change in P(r) thus can still be a post-s mechnism
2. Blockade of NMDArs
  1. Apply MK-801 -use-dependent, irreversible NMDAr blocker
    1. Can measure how quickly the NMDAr is blocked
    2. Higher rate of block = increase in P(r) = pre S mechanism
3. Liu and Tsien (1990)
Synaptic efficacy
1. Size of response every time you get one - failures and successes
  1. time-averaged Post-S response
Synaptic potency
1. Average size of response when there is one
If P(r) = 1 then potency = efficacy
1. If P(r)< 1 then efficacy<potency
P(r) studies
1. Minimal stimulation + patch clamp recording
  1. Do paired pulse stimulation and can measure No. of FAILURES!!
    1. If rate of failures changes then P(r) must change - preS mechanism

Zusammenfassung der Ressource

	Erstellt von ob8313 vor etwa 11 Jahre