449175
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Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor mehr als 10 Jahre
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Frage 1
Frage
Define Protein Folding
Antworten
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The process by which a protein obtains its natural 3D state
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The process by which a polypeptide chain is created from mRNA
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The process by which DNA sequences are converted into an mRNA chain
Frage 2
Frage
Which structure of a protein shows biological activity?
Antworten
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Primary
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Secondary
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Tertiary
Frage 3
Frage
Which of the following occurs to ALL proteins?
Antworten
-
Non-covalent folding
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Covalent folding
Frage 4
Frage
Which of the following are covalent modifications of the polypeptide chain?
Antworten
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Glycosylation
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Phosphorylation
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Formation of disulphide bonds
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Van Der Waals interactions
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Electrostatic interactions
Frage 5
Frage
Folding corresponds to a hierarchy of protein structure
Antworten
- True
- False
Frage 6
Frage
Put the following in Order
A -Unfolded
B - Nucleation
C - Secondary Structure
D - Domain
E - Active Oligomer
F - Inactive Oligomer
G - Supersecondary Structure
H - Folded Monomer
Antworten
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A-B-C-G-D-H-F-E
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A-B-E-F-G-D-C-H
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A-D-C-B-E-F-H
Frage 7
Frage
What is meant by the 'Thermodynamic Hypothesis of Protein Folding'
Antworten
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That proteins fold into different structures based on the energy available to them in the medium
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That the information for correct protein folding is found within the sequence itself
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That the protein will always fold into the lowest possible energy state
Frage 8
Frage
The Kinetic Hypothysis suggests that there is a specific folding pathway for each protein- protein folding is not random
Antworten
- True
- False
Frage 9
Frage
Describe the features of the RNAase Refolding experiment?
Antworten
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RNAase is denatured with urea
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RNAase is denatured with detergent
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And an oxidising agent to remove the disulphide bridges
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And a reducing agent to remove the disulphide bridges
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Once the denaturing agents were removed and it was re-oxdised
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Once the denaturing agents were removed and it was re-reduced
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Protein fully reformed itself and worked
Frage 10
Frage
How do we know the RNAase really was denatured during the experiment?
Antworten
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If the protein was allowed to reform before the reducing agents were removed, the sequence showed non native sulphides and didn't work
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We don't know- we can never really KNOW anything
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If the protein was allowed to reform before the reducing agents were removed, the sequence didn't work
Frage 11
Frage
Which of the following are conditions for In Vitro Refolding (Like in the RNAase experiment)?
Antworten
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Structure must be amenable to environmental changes
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Free energy path must be relatively smooth
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Must contain covalent bonds
-
Must have slow transitions
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Structure must have unique free energy
Frage 12
Antworten
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Cis
-
Trans
Frage 13
Frage
Trans formations are less stable due to their steric hindrance
Antworten
- True
- False
Frage 14
Frage
Proline is added to an amino acid chain exclusively in what form?
Antworten
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Trans
-
Cis
-
The other form occurs 10-40% of the time, and must be altered post addition
Frage 15
Frage
Which of the following proteins converts cis isomers to their trans form?
Antworten
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Peptidyl Propyl Isomerases
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Peptidyl Glucyl Isomerases
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Peptidyl Lipyl Isomerases
Frage 16
Frage
In the Cis form, the clash of functional groups around the alpha carbon is much greater due to steric hindrance
Antworten
- True
- False
Frage 17
Antworten
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Disulphide
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Van Der Waals
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Electrostatic Attraction
Frage 18
Frage
The previous amino acid to proline experiences steric hindrance in either proline conformation
Antworten
- True
- False
Frage 19
Frage
Cis proline has _ energy than/to trans proline
Antworten
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Greater
-
Less
-
Equal
Frage 20
Frage
The two cystine molecules that bind together are known as what?
Antworten
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Cysteine
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Bi-cystine
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Bi-sulphur cystine
Frage 21
Frage
Proteins with di-sulphide bonds are most likely to be found where?
Antworten
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In low/high pH
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Extracellular proteins
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Cytoplasmic proteins
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In low/high salt concentrations
Frage 22
Frage
Phosphorylation occurs on which residues?
Antworten
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Serine
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Valine
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Threonine
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Tyrosine
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Glutamine
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Asparagine
Frage 23
Frage
Pro-sequences are found at the C-terminus
Antworten
- True
- False
Frage 24
Frage
The Pro-sequence is also known as
Antworten
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A chaperone sequence
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A ligation sequence
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A glycosylation sequence
Frage 25
Frage
Which proteins will have a pro-sequence?
Antworten
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Chaperones
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Proteases
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Ligand-binding proteins
Frage 26
Frage
The protein with a pro-sequence will auto-cleave it before folding correctly
Antworten
- True
- False
Frage 27
Frage
What is co-operativity in protein folding?
Antworten
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Where one area of the protein that has already folded provides a nucleation site for another domain to fold
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The pre-formed proteins will be used as a template to fold more of that species
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Where chaperone proteins assist in protein folding
Frage 28
Frage
What does the Hydrophobic Zipper Model suggest?
Antworten
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That hydrophobic amino acids in close proximity are more likely to interact
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That hydrophillic amino acids in close proximity are more likely to interact
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Constraining the chain and making other residues more likely to interact, which further constrains the chain, etc.
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