453902
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace más de 10 años
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Pregunta 1
Pregunta
What is required for the protein surface to bind to the DNA surface?
Respuesta
-
For the protein surface to be complimentary to the DNA surface
-
For the protein surface to be positive (DNA backbone's are negative)
-
For the protein surface to contain hydrophobic residues
Pregunta 2
Pregunta
In which groove can the order of base pairs be differentiated?
Respuesta
-
Major groove
-
Minor groove
Pregunta 3
Pregunta
The major groove is 15A wide
Respuesta
- True
- False
Pregunta 4
Pregunta
Which is smaller?
Respuesta
-
Major groove
-
Minor groove
Pregunta 5
Pregunta
Proteins that bind in the major groove must be more specific than those that bind in the minor groove
Respuesta
- True
- False
Pregunta 6
Pregunta
Why is Arg such a useful residue?
Respuesta
-
It has 2 H acceptors, so it can bind to guanine
-
It has 2 H donors, so it can bind to guanine
-
It has 2 H acceptors, so it can bind to cytosine
Pregunta 7
Pregunta
Which of the following can bind to both G & A
Respuesta
-
Glu
-
Val
-
Asn
-
Tyr
Pregunta 8
Pregunta
What is base stacking?
Respuesta
-
Where hydrophobic bases stack to avoid water
-
Where hydrophobic bases pull the DNA inwards to avoid water
-
Where hydrophobic bases twist the DNA to avoid water
Pregunta 9
Pregunta
What is required for a protein to take advantage of base stacking?
Respuesta
-
Residues with delocalised electrons
-
Hydrophobic residues
-
Hydrphillic residues
-
For it to make a gap between bases
Pregunta 10
Pregunta
10bp covers a distance of 34 A
Respuesta
- True
- False
Pregunta 11
Pregunta
Describe DNA
Respuesta
-
Long, repetitious and thin
-
Long, non-repetitious and thick
-
Short, repetitious and thin
-
Long, repetitious and thick
-
Short, non-repetitious and thin
Pregunta 12
Pregunta
DNA can bend 180 dgrees over how long a stretch?
Respuesta
-
40
-
30
-
20
Pregunta 13
Pregunta
What does Phage Lamda Repressor bind to?
Respuesta
-
Phage DNA
-
Bacterial DNA
Pregunta 14
Respuesta
-
A half site
-
A palindromic sequence
-
A restriction site
Pregunta 15
Pregunta
How does Phage Lambda Repressor Work?
Respuesta
-
Binds to bacterial DNA at the lambda cro gene
-
Binds to phage DNA at the Lambda cro gene
-
And blocking RNA polymerase binding
Pregunta 16
Pregunta
Phage Lambda Repressor binds to only one target sequence
Respuesta
- True
- False
Pregunta 17
Pregunta
The Phage Lambda repressor binds as a _
Respuesta
-
Monomer
-
Dimer
-
Tetramer
Pregunta 18
Pregunta
Each subunit attaches to the DNA using a _ and binds to the other subunits using a _
Respuesta
-
Alpha helix, alpha helix
-
Alpha helix, nothing- it's a monomer
-
Beta sheet, beta sheet
-
Beta sheet, nothing- it's a monomer
Pregunta 19
Pregunta
Phage Lambda Repressor has multiple subunits because ...
Respuesta
-
It binds to a sequence long enough that the protein cannot be removed
-
It binds to a sequence long enough that the sequence becomes unique int the DNA
-
It binds more exactly - so it only has a single target sequence
Pregunta 20
Pregunta
Where does Phage Lambda Suppressor bind?
Respuesta
-
2 adjacent major grooves
-
2 adjactent minor grooves
-
An adjactent major & minor groove
-
32A apart
-
34A apart
-
36A apart
Pregunta 21
Pregunta
A non-specific interaction doesn't involve bonding with any particular base
Respuesta
- True
- False
Pregunta 22
Pregunta
List some specific interactions between Phage Lambda Repressor and DNA
Respuesta
-
Ser - G, and Gly - T,
-
Gly- T and Ser-G
-
Gly- C- and Ser-T
Pregunta 23
Pregunta
The DNA sequence is all read from the same DNA strand
Respuesta
- True
- False
Pregunta 24
Pregunta
A helix-turn-helix motif is often used for reading DNA
Respuesta
- True
- False
Pregunta 25
Pregunta
Different alpha helices (of the helix-turn-helix) motif perform specific and non-specific interactions
Respuesta
- True
- False
Pregunta 26
Pregunta
Proteins don't have to land on the DNA in exactly the right spot, they only need to...
Respuesta
-
Bind to the phosphate backbone
-
Bind to the bases
-
And then roll freely along the DNA
Pregunta 27
Pregunta
Methionine repressor...
Respuesta
-
prevents methionine production
-
prevents binding of methionine to tRNA
-
prevents methionine being added to the amino acid chain
Pregunta 28
Pregunta
The Methionine Repressor binding site is...
Respuesta
-
Palindromic
-
Almost palindromic
-
8bp
-
16bp
Pregunta 29
Pregunta
The binding site of most helix-turn-helix proteins are 16-18bp long
Respuesta
- True
- False
Pregunta 30
Pregunta
How does the Methionine repressor bind to the DNA?
Respuesta
-
Uses 2 alpha helices
-
Uses 2 beta sheets
-
One from each monomer
-
One from each end of the monomer
-
Into one major groove
-
Into two major grooves
Pregunta 31
Pregunta
Two beta strands have the same diameter as an alpha helix
Respuesta
- True
- False
Pregunta 32
Pregunta
Specific interactions in the methionine repressor happen between Lysine, Threonine, A & G
Respuesta
- True
- False
Pregunta 33
Pregunta
Restrictions enzymes are part of the bacterial immunity... how?
Respuesta
-
Recognises and destroys non-self DNA
-
Cuts around viral DNA that has infected the genome
-
Proteases break down the viral coat
Pregunta 34
Pregunta
Restriction enzymes and transferases come in pairs, with the restriction enzyme made first
Respuesta
- True
- False
Pregunta 35
Pregunta
How does EcoRI bind?
Respuesta
-
Phosphate backbone reactions in the minor groove
-
Phosphate backbone reactions in the major groove
-
Specific interactions in the minor groove
-
Specific interactions in the major groover
Pregunta 36
Pregunta
EcoRI looks for 2 G-C on top of each other because they're more bendable
Respuesta
- True
- False
Pregunta 37
Pregunta
Where in the GAATTC sequence does EcoR1 cut?
Respuesta
-
After the C, before the C
-
After the A & T
-
In the middle
Pregunta 38
Pregunta
Eco RI needs an Fe iron to bind
Respuesta
- True
- False
Pregunta 39
Pregunta
Where are the distortions of the DNA caused by EcoRI?
Respuesta
-
In the centre of GAATTC
-
G/AATT/C
-
GA/AT/TC
Pregunta 40
Pregunta
Hydrophobic interactions in ECORI-DNA ineractions recognise what?
Respuesta
-
AA atacking
-
AT stacking
-
TT stacking
Pregunta 41
Pregunta
ECORI uses water mediated interactions to assist DNA recognition
Respuesta
- True
- False
Pregunta 42
Pregunta
What is strange about the EcoRV restriction enzyme?
Respuesta
-
It has an abnormally large binding site
-
It leaves blunt ends
-
It binds to 2 different sequences
Pregunta 43
Pregunta
EcoRV forms a dimer
Respuesta
- True
- False
Pregunta 44
Pregunta
ECORV creates major distortions where?
Respuesta
-
In the middle
-
At the ends
-
One residue in
Pregunta 45
Pregunta
ECORV binds to the backbone in the minor groove and wraps 'arms' around into the major groove
Respuesta
- True
- False
Pregunta 46
Pregunta
The recognition loops in the major groove cause...
Respuesta
-
H bonding between stacks
-
H bonding between A-G
-
H bonding between T-C
Pregunta 47
Pregunta
ECORV looks for ATAT bases
Respuesta
- True
- False
Pregunta 48
Pregunta
Where in the GCGC sequence does M.Hal methylate?
Respuesta
-
The first G
-
The first C
-
The second G
-
The last C
Pregunta 49
Pregunta
Methylation prevents the restriction enzyme from recognizing a sequence, so it cannot be cot
Respuesta
- True
- False
Pregunta 50
Pregunta
Describe the actions of methylases
Respuesta
-
Methylate both strands at once
-
Methylate one strand at a time
-
Are monomers
-
Are dimers
Pregunta 51
Pregunta
Interactions 'pull out' one of the bases to gain chemical access
Respuesta
- True
- False
Pregunta 52
Pregunta
What is the donor for methylation?
Respuesta
-
S-adenosyl-methionine
-
Methanol
-
Methide
Pregunta 53
Pregunta
How the methylation interaction studied?
Respuesta
-
The cystine had the 5th H replaced with an F
-
Trapping it in the active site
-
The cystine had the 3rd H replaced with an F
Pregunta 54
Pregunta
Once in the active site, a thiol bond forms between the cystine and the cytosine so the methyl can be added
Respuesta
- True
- False
Pregunta 55
Pregunta
What is inserted to bind with the widowed G?
Respuesta
-
Glu
-
Arg
-
Cys
Pregunta 56
Pregunta
The inserted bases fill up the space left by the flipped out cytosine
Respuesta
- True
- False
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