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Description
Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312
over 10 years ago
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| Question | Answer |
| Types of Heat Shock Proteins in Prokaryotes | Cytoplasmic & Periplasmic |
| Types of Heat Shock Proteins in Eukaryotes | Cytoplasmic, Nuclear, ER & Perinuclear |
| HSP Size range | 14-40 kDa |
| HSP reguation | Phosphorylation |
| Normal Role of Hsp27 | Stabilisation of actin |
| Phosphorylation of Hsp27 | Decreases size of oligomers- keeps it in small, probably dimeric form |
| Hsp27 in cell under stress | Are dephosphorylated and form mutimers (700-800kDa) |
| Role of Hsp27 in cell under stress | Binds to unfolded protein to store for refolding |
| Structure of Hsp16.9 | Dodedecamer- 2 layers of 6 monomers |
| Dimensions of Hsp16.9 | 120x65A |
| Role of HSP's | Bind to unfolding proteins and stabilise them for refolding later |
| Immunoglobulin Domain | |
| Structure of Ig domain | 2 layers of aligned beta sheets (6-9 stands each) with a Greek key motif |
| Dimerisation of Ig domain | Allows dimerisation of Hsp's |
| What is PapD? | A bacterial homologue of Hsp's |
| Location of PapD | Periplasmic Space |
| Role of PapD | Pilus Construction |
| Prevention of Innapropriate Pap_ Interactions | Pap F, E, K, A, H, G don't have a complex Ig domain, which must be completed by PapD's |
| Chaperone Donor Strand Complimentation in Pap Proteins | PapD donates a beta sheet to the others to complete their Ig folds, allowing them to react only when they're supposed to |
| Site of PapD donation | Missing bets strand forms a groove of sites P2-5- PapD's beta binds to P2-5 |
| Nte | Contained by all Pap proteins but PapG & allows Pap proteins to bind together |
| Donor Strand Exchange | |
| Role of P5 Residue/Pocket | With PapD bound, P5 of incoming Pap protein binds to P5 pocket of PapD bound protein |
| Once Donor Strand Complimentation is complete | The binding Pap protein competes out PapD and binds to the other Pap protein using it's Nte domain |
| Pap protein competitiveness | The sequence of Pap proteins determines their affinity for others, which determines where in the Pili sequence they go |
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