Heat Shock Proteins

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Flashcards by gina_evans0312, updated more than 1 year ago
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Structural Basis for Biological Function (Protein Folding) Flashcards on Heat Shock Proteins, created by gina_evans0312 on 21/12/2013.

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Question Answer
Types of Heat Shock Proteins in Prokaryotes Cytoplasmic & Periplasmic
Types of Heat Shock Proteins in Eukaryotes Cytoplasmic, Nuclear, ER & Perinuclear
HSP Size range 14-40 kDa
HSP reguation Phosphorylation
Normal Role of Hsp27 Stabilisation of actin
Phosphorylation of Hsp27 Decreases size of oligomers- keeps it in small, probably dimeric form
Hsp27 in cell under stress Are dephosphorylated and form mutimers (700-800kDa)
Role of Hsp27 in cell under stress Binds to unfolded protein to store for refolding
Structure of Hsp16.9 Dodedecamer- 2 layers of 6 monomers
Dimensions of Hsp16.9 120x65A
Role of HSP's Bind to unfolding proteins and stabilise them for refolding later
Immunoglobulin Domain
Structure of Ig domain 2 layers of aligned beta sheets (6-9 stands each) with a Greek key motif
Dimerisation of Ig domain Allows dimerisation of Hsp's
What is PapD? A bacterial homologue of Hsp's
Location of PapD Periplasmic Space
Role of PapD Pilus Construction
Prevention of Innapropriate Pap_ Interactions Pap F, E, K, A, H, G don't have a complex Ig domain, which must be completed by PapD's
Chaperone Donor Strand Complimentation in Pap Proteins PapD donates a beta sheet to the others to complete their Ig folds, allowing them to react only when they're supposed to
Site of PapD donation Missing bets strand forms a groove of sites P2-5- PapD's beta binds to P2-5
Nte Contained by all Pap proteins but PapG & allows Pap proteins to bind together
Donor Strand Exchange
Role of P5 Residue/Pocket With PapD bound, P5 of incoming Pap protein binds to P5 pocket of PapD bound protein
Once Donor Strand Complimentation is complete The binding Pap protein competes out PapD and binds to the other Pap protein using it's Nte domain
Pap protein competitiveness The sequence of Pap proteins determines their affinity for others, which determines where in the Pili sequence they go
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