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E. coli strain growth in PCA agar

Growth of work culture in TSB (at 35 ºC for 18 h)

Serial dilution of the obtained growth (initial 10^3 to 10^9 CFU/mL)

Spread of diluted cultures on PCA plates (10^2, 10^3, 10^4, 10^5 and 10^6 UFC/plate inoculums).

Exposure of contaminated plates by 0.5, 1, 2, 3, 4 and 5 minutes (+ control) in two operational scenarios.

Incubation of the plates at 37 ºC for 24 h.

Counting of the growths obtained

Kinetic analysis, adjustment of models to microbial inactivation curves

Evaluation of results