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Sin título
Fichas del conjunto (43)
- 1—Alcian Blue, pH 1.0
- 2Alcian Blue, pH 1.0 Reagents used (4)0.1N HCl acid rinse
1% Alcian blue w/ HCl acid
0.1N HCL acid rinse
Nuclear fast red (NFR) - 3Alcian Blue, pH 1.0 Purposedemonstration of sulfated mucocubstances
- 4Alcian Blue, pH 1.0 Fixation (2)10% NBF
Bouin's solution - 5Alcian Blue, pH 1.0 control tissuecolon, small intestine, appendix
- 6Alcian Blue, pH 1.0 resultsSulfated GaGs and sulfomucins - pale blue
Background - pink to red - 7Alcian Blue, pH 1.0 troubleshooting: 1. Excessive background 2. Blue nuclear staining 3. Weak staining 4. Cloudiness1. Rinse with acetic acid wasn’t sufficient; pH wasn’t maintained;
2. Blue solution step too long
3. Dye solution is weak or old; Poor hydration step
4. Insufficient washing after NFR - 8—Alcian Blue, pH 2.5 w/ Hyaluronidase
- 9Alcian Blue, pH 2.5 w/ Hyaluronidase reagents used (4)Hyaluronidase
3% acetic acid
1% Alcian blue w/ acetic acid
3% acetic acid
Nuclear fast red (NFR) - 10Alcian Blue, pH 2.5 w/ Hyaluronidase PurposeDifferentiates between epithelial cells (acidic glycoproteins) and connective tissue (GaGs); carcinoma vs mesothelioma
- 11Alcian Blue, pH 2.5 w/ Hyaluronidase PrincipleHyaluronidase digestion results in cleavage of glycosidic linkages and chondroitin; Absence of dramatically reduced staining indicates
- 12Alcian Blue, pH 2.5 w/ Hyaluronidase PrincipleHyaluronidase digestion results in cleavage of glycosidic linkages and chondroitin; Absence of dramatically reduced staining indicates
- 13Alcian Blue, pH 2.5 w/ Hyaluronidase Fixation (2)10% NBF
Bouin's solution - 14Alcian Blue, pH 2.5 w/ Hyaluronidase Control tissue2 slides of umbilical cord, intestine or appendix can be used as a 2nd control to demonstrate acidic glycoproteins
- 15Alcian Blue, pH 2.5 w/ Hyaluronidase resultsBackground — pink
Without digestion — deep blue
With digestion (GaGs and chondroitin will be absent) — loss of staining - 16Alcian Blue, pH 2.5 w/ Hyaluronidase Troubleshooting: 1. excessive background 2. blue nuclear staining 3. weak staining 4. cloudiness1. rinse with acetic acid wasn’t sufficient; pH wasn’t maintained
2. Blue solution kept too long
3. Dye solution is weak or old; Poor hydration step
4. insufficient washing after nuclear fast red - 17—Alcian Blue, pH 2.5
- 18Alcian Blue, pH 2.5 Reagents used (3)3% acetic acid rinse
1% Alcian blue w/ 3% acetic acid
3% acetic acid rinse
Nuclear fast red (NFR) - 19Alcian Blue, pH 2.5 Purposedemonstration of Gags and acidic glycoproteins (sialomucins and sulfomucins); differentiated by different shades of blue; more specific than colloidal iron
- 20Alcian Blue, pH 2.5 PrinciplepH 2.5 is important because sulfated and carboxylated mucopolysaccharides and glycoproteins will all stain
- 21Alcian Blue, pH 2.5 Fixation (2)10% NBF
Bouin's solution - 22Alcian Blue, pH 2.5 Control tissueColon, small intestine, appendix
- 23Alcian Blue, pH 2.5 resultsAcidic glycoproteins — blue (more intense blue)
GaGs — light blue
Background & nuclei — pink to red - 24Alcian Blue, pH 2.5 Troubleshooting: 1. Excessive background 2. Blue nuclear staining 3. Weak staining 4. Cloudiness1. rinse with acetic acid wasn’t sufficient; pH wasn’t maintained
2. blue solution step too long
3. dye solution is weak or old; poor hydration step
4. insufficient washing after NFR - 25—Alcian Blue -- PAS
- 26Alcian Blue -- PAS reagents used (5)3% acetic acid
1% alcian blue w/ acetic acid
0.5% periodic acid
schiff reagent
hematoxylin - 27Alcian Blue - PAS purposeDifferentiate neutral and acidic glycans; Acidic mucosubstances are stained with Alcian blue; Neutral substances are stained with PAS
- 28Alcian Blue - PAS fixation (2)10% NBF
Bouin's - 29Alcian Blue - PAS control tissuekidney or cervix
- 30Alcian Blue - PAS results- GaGs & acidic glycoproteins: blue
- Neutral mucosubstances & glycogen: magenta
- Nuclei: purple - 31—Alcian Yellow — toluidine blue
- 32Alcian Yellow — toluidine blue reagents used (4)- periodic acid
- potassium/sodium metabisulfite
- alcian yellow
- T. blue - 33Alcian Yellow — toluidine blue purposestains H. Pylori
- 34Alcian Yellow — toluidine blue fixation10% NBF
- 35Alcian Yellow — toluidine blue control tissueKnown H. Pylori positive tissue
- 36Alcian Yellow — toluidine blue results- H. Pylori: blue
- Mucin: yellow
- Background: pale blue - 37—Aldehyde Fuchsin Elastic Stain
- 38Aldehyde Fuchsin Elastic Stain reagents used (3)- aldehyde fuchsin solution
- 70% alcohol
- light green or van gieson - 39Aldehyde Fuchsin Elastic Stain purposeto demonstrate elastic fibers
- 40Aldehyde Fuchsin Elastic Stain fixation10% NBF
acid chromates - 41Aldehyde Fuchsin Elastic Stain control tissueaorta, skin, muscular artery
- 42Aldehyde Fuchsin Elastic Stain results- elastic fibers: deep blue to purple
- background: green - 43Aldehyde Fuchsin Elastic Stain troubleshooting: 1: inadequate elastic staining 2: poor contrast1: old/overused solution
2: overpowering counterstain
Información
- Descripción
- HTL Histology Fichas sobre Sin título, creado por Sydney Hanula el 15/10/2023.
- Creado por
- Sydney Hanula
- Visitas
- 2
- Valoración