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S.08.01

Zoe Fernyhough Inglés 15 fichas

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1Mb functionMuscle cells
transports O2 from cytosol to mit.
2Hb functioncarries O2 in RBCs, from lungs to tissues
carries H+ and CO2 from tissues to lungs
3how much of Erythrocytes are Hb?30%, increasing O2 solubility in blood by 98%
4RBC energyno mitochondria/ other orgnelles so..
Anaerobic glycolysis, PPP,
5Conjugated protein = apoprotein + cofactor (for Hb and Mb)Mb: apoMb + 1 Heme group
Hb: 2 alpha, 2 beta chains + 4 Hemes
6Hb structureEach Hb: 4 subunits: 1 alpha or beta, attached to a Heme
Tetrahedral arrangement
7Ferric v FerrousFerric: Fe 3+ (oxidized) (hemin)
Ferrous: Fe 2+ (reduced) (heme)
8Heme structurePlanar, aromatic, Fe in middle attached to 4 Ns, mostly nonpolar
93 interactions within Heme moleculenonpolar pocket for heme
covalent bond: heme and iron
ion pairing: COO- w/ + charged AA
10Where does O2 bind in Hb and Mb?Fe in 6th coordination spot
H bonds to His
11Oxygenation vs. OxidationOxygenation: Heme binds O2
Oxidation: Heme --> Hemin + O2. reactive
12metHb/ methemoglobinemiametHb build up, (low O2 transport) treatable w/ reducing agents
13Mb Structure75% is alpha helices (8)
similar to globin folds
nonpolar AAs interior
14Heme binding in Mbnonpolar side chains
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