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- 1EfficiencyThe efficiency of a chromatographic peak is a measure of the dispersion of the analyte band as it travels through the HPLC system and column.
The plate number (N) is a measure of the peak dispersion on the HPLC column, which reflects the column performance.
Each plate is the distance over which the sample components achieve one equilibration between the stationary and mobile phase in the column. - 2Selectivitythe ability of the chromatographic system to ‘chemically’ distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the apices of the two peaks.
a = k2/k1 = (tr2-t0)/(tr1-t0) - 3Retention (Capacity) Factor (k)means of measuring the retention of an analyte on the chromatographic column.
k=(tr-t0/t0)
high k value indicates
that the sample is highly
retained and has spent a
significant amount of time
interacting with the
stationary phase. - 4ResolutionResolution is calculated using the separation of two peaks in terms of their average peak width at the base (tR2 > tR1).
resolution value of 1.5 or greater between two peaks will ensure that the sample components are well (baseline) separated to a degree at which the area or height of each peak may be accurately measured.
Rs = efficiency x retention x selectivity - 5How To Change Selectivity1. pH
2. Organic Solvent
3. M. Phase additives
4. Stationary Phase
5. Temperature - 6Peak Capacityhg
- 7Tailing Factorhg
- 8Linear Velocity
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- Fichas sobre Sin título, creado por Matt Boag el 14/11/2016.
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- Matt Boag
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