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SG lab 3 quiz
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- 1What was the goal of lab 3?ligate the DNA fragments produced during Lab 2, using DNA ligase to make new recombinant plasmids (pARA-R)
- 2What was the goal plasmid to make during this lab? Indicate the important features of this particular plasmid and the function of each feature.pARA-R
- ampicillin resistance (ampR): allows for identification of bacteria that have taken in the plasmid
- rfp gene
- promoter sequence (pBAD): initiating transcription
- arabinose activator sequence (araC):
- ori sequence: initiating DNA replication - 3What is the role of DNA Ligase in replication? What about in gene cloning?- replication: one of the daughter strands is made up of DNA fragments and the ligase joins the fragments by catalyzing the formation of covalent bonds between adjacent nucleotides
- gene cloning: the ligase catalyzes bonding along DNA's sugar-phosphate backbone after the bases in complementary sticky ends have formed hydrogen bonds to each other - 4Why did we need to inactivate BamHI and HindIII before beginning this lab?otherwise it would cut/denature the DNA, when the goal of the lab is to ligate the fragments
- 5What was in the LIG tube? What happened in this tube?pKAN-R fragments, pARA-R fragments, buffer, and water
- 6What properties of the DNA restriction fragments allow for ligation to occur?complementary sticky ends; the ligase catalyzes bonding of the backbone only after the base pairs have been created
- 7Could two rfp-pBAD fragments form during ligation? Why or why not?Yes, the two fragments can join because they have complementary sticky ends
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- ap bio lab 3 quiz study guide
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- Sophia Khan
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