HPLC

HPLC

1. Small Quantities & Sensitive Detector

   • Solid Stationary Phase
   • Liquid Mobile Phase

2. Uses high pressure to force solvent through closed columns containing fine particles

3. GC is better but some compounds aren't volatile enough

4. Chromatographic Process

   • Increasing rate at which solute equilibrates through phases increases efficiency
   • Small particles give high efficiency but need high pressure
     • Efficiency increases in a packed column as size of S phase particles decrease
     • 1.7 - 5 μm
     • Van Deemter
       • Shows plate height vs. linear flow rate
       • Small particles reduce plate height and isn't very sensitive to increased flow rate