CHROMATOGRAPHY

CHROMATOGRAPHY

1. method of separating 2 or more components that dissolve in the same solvent

2. Paper Chromatography

   • solvent travels up the chromatography paper (chromatogram)
   • Rf values
     • Rf value= distance travelled by substance/ distance travelled by solvent
     • constant ratio (retention factor of substance)

3. Chromatography of colourless substances

   • spray locating agent on chromatogram
     • Causes the colourless substances to show up as coloured spots
   • e.g. amino acids

4. Uses

   • separate components in a sample
   • identify components in a sample
   • determine whether a sample is pure