Week 4: Microscopy and Staining

1. 1What is the charge of the outside of the bacterial cell?

   1. ANegative
   2. BPositive
2. 2What is the charge of the stain in simple staining?

   1. APositive
   2. BNegative
3. 3Which type of stain binds to the cell walls of an organism?

   1. ABasic staining
   2. BAcidic staining
4. 4Sort the stains by their charge. They are in alphabetical order under their corresponding stain type.
   
   Simple/Basic Stain
   [blank_start]crystal violet[blank_end]
   [blank_start]methylene blue[blank_end]
   [blank_start]safranin[blank_end]
   
   Negative/Acidic Stain
   [blank_start]congo red[blank_end]
   [blank_start]India ink[blank_end]

   1. Acrystal violet
   2. Bmethylene blue
   3. Csafranin
   4. Dcongo red
   5. EIndia ink
5. 5Name of stain: What is differentiated?
   [blank_start]Gram[blank_end]: cell wall characteristics
   capsule: formation of [blank_start]capsule[blank_end]
   acid fast: [blank_start]cell wall characteristics[blank_end]
   endospore: formation of [blank_start]endospores[blank_end]
   

   1. AGram
   2. Bcell wall characteristics
   3. Ccapsule
   4. Dendospores
6. 6A Gram-Positive cell will stain purple.

   1. AVerdadero
   2. BFalso
7. 7Gram Stain:
   Primary stain: [blank_start]crystal violet[blank_end]
   Decolorizer: [blank_start]ethanol[blank_end]
   Counterstain: [blank_start]Safranin[blank_end]
   Positive result: [blank_start]Purple[blank_end]
   Negative result: [blank_start]Pink[blank_end]

   1. Acrystal violet
   2. Bethanol
   3. CSafranin
   4. DPurple
   5. EPink
   6. Fmalachite green
   7. Gacid/alcohol
   8. Hacetone
   9. Imethylene blue
   10. Jblue
   11. Kgreen
8. 8Acid-fast Stain:
   Primary stain: [blank_start]carbolfushin[blank_end]
   Decolorizer: [blank_start]acid-alcohol[blank_end]
   Counterstain: [blank_start]methylene blue[blank_end]
   Positive result: [blank_start]pink[blank_end]
   Negative result: [blank_start]blue[blank_end]

   1. Acarbolfushin
   2. Bacid-alcohol
   3. Cmethylene blue
   4. Dpink
   5. Eblue
   6. Fgreen
   7. Gred
   8. Hmalachite green
   9. Isafranin
   10. Jcrystal violet
   11. Kethanol
9. 9Capsule Stain:
   Primary stain: [blank_start]congo red[blank_end]
   Counterstain: [blank_start]Maneval's stain[blank_end]
   Positive result: [blank_start]clearing/white halo[blank_end]
   Negative result: [blank_start]stained[blank_end]

   1. Acongo red
   2. BManeval's stain
   3. Cclearing/white halo
   4. Dstained
   5. Egreen spore
   6. Fsafranin
   7. Gmethylene blue
   8. Hred
   9. Ipink

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Test sobre Week 4: Microscopy and Staining, creado por Amanda A el 27/02/2019.

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Amanda A

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