Bacteria and Archaea

1. 1tick the primary identification tests of bacteria

   1. Ahow the colony looks to the eye (morpthology, borders etc)
   2. Bmicroscopic observations ( gram stain , motility , endospores present or not etc)
   3. Ccatalyse test
   4. Doxidase test
2. 2label the binary fission diagram

   

   1. Acell wall
   2. Bnucleoid
   3. Ccytoplasmic membrane
   4. Dreplicated dna
   5. Ecell replicates DNA
   6. FDaughter cells
   7. GCross wall forms completely
   8. Hcross wall begins to form
   9. Imembrane invaginates ( fold onto itself)
   10. Jmembrane stretches separating DNA
3. 3label the main groups of gram negative bacteria organised by characteristics

   

   1. Abacteroides
   2. Benterobacteria
   3. Cvibro
   4. Dneisseria
   5. Eacinetobacter
   6. Fpsudeomonas
   7. Gazotobacter
   8. Hlegionella
4. 4label the gram positive bacteria based on their characteristics

   

   1. Abacillus
   2. Bclostridium
   3. Clisteria
   4. Dmycobacterium
   5. Elactobacillus
   6. Fmicrococcus
   7. Gstaphylococcus
   8. Hstreptococcus
   9. Ileuconostoc
   10. Jsarcina
5. 5match the following
   [blank_start]MEDIUM[blank_end] - nutrient solution for growing microorganisms
   [blank_start]INOCULUM[blank_end] - initial small number of cells added and grown to give a CULTURE
   [blank_start]AGAR[blank_end] - polysaccharide extracted from seaweed that melts at 100 degrees remains molten down to 50 degrees
   

   1. AMEDIUM
   2. BINOCULUM
   3. CAGAR
6. 6match the catergories of media to their descrpitions
   [blank_start]Complex[blank_end] - undefined reagents ( chemical compo is unknown)
   [blank_start]Enriched[blank_end] - contain the nutrients required to support the growth of a wide variety of organisms
   [blank_start]Selective[blank_end] - agent only allows growth of certain organisms
   [blank_start]Differential[blank_end] - agent changed in some visible way
   [blank_start]Synthetic[blank_end] - defined, Analar reagents ( chemical compo is known)

   1. AComplex
   2. BEnriched
   3. CSelective
   4. DDifferential
   5. ESynthetic
7. 7tick the following selective agents used in media

   1. Asalt
   2. Bcystal violet
   3. Cbile salts
   4. Dchloramphenical
   5. Eneomycin
   6. Fblood
   7. Gfermentable carbs
8. 8tick the differential agents

   1. Ablood
   2. Bfermntable carbs and Ph indicator
   3. Cgas production
   4. Dsalt
   5. Ebile salts
   6. Fneomycin
9. 9what are the charcteristics f Archaea

   1. Alive in extreme environments ( heat vents etc)
   2. Bmembranes have ether linkages
   3. C16S rRna signature sequence
   4. Dlive in easy environments ( nutritious soil)
10. 10tick the similarietes archaea have with bacteria

    1. Asize and shape
    2. Bprokaryotic cell organisation
    3. Cribosome size
    4. Dno peptidoglycan in cell wall
    5. Einsensitivity to antibiotics

11. 11tick the differences archae have to bacteria

    1. Ano peptidoglycan in cell wall
    2. Bcell membrane (no ester linkages)
    3. Ccomplex RNA polymerase (10-13 subunits)
    4. Dinsensitivity to antibiotics
    5. Eribosome size
    6. Fprokaryotic cell organisation
12. 12what is/are the currently accepted phyla ( levlle of classicfication)

    1. ACrenarchaeota (e.g Hyperthermophiles- heat loving)
    2. BEuryarchaeota (e.g Extreme halophiles - salt loving & methanogens- methane makers)
    3. CThaumarchaeota (e.g ammonia oxidising)
    4. DAll of the following
    5. ENone of the following
13. 13the [blank_start]optimum tempo[blank_end] for Hyperthermophiles is [blank_start]above 80 degrees[blank_end]. they can be isolated from [blank_start]hot springs[blank_end] and [blank_start]hyrothermal vents[blank_end] and are a prevalent source of [blank_start]thermostable enzymes[blank_end]

    1. Aoptimum temperature
    2. Babove 80 degrees
    3. Cbelow 80 degrees
    4. Dhot springs
    5. Ehyrothermal vents
    6. Fthermostable enzymes
14. 14[blank_start]methanogens[blank_end] are all [blank_start]obligate methane producers[blank_end] who get their energy by [blank_start]synthesise methane[blank_end]. they are found mostly in [blank_start]anaerobic environments[blank_end] like animal guts or waterlogged soil. the use [blank_start]CO2[blank_end] as substrates for [blank_start]anaerobic respiration[blank_end]

    1. Amethanogens
    2. Bobligate methane producers
    3. Csynthesising methane
    4. Danaerobic environments
    5. ECO2
    6. Fanaerobic respiration
15. 15many extreme halophiles contain bacteriorhodopsin

    1. AVerdadero
    2. BFalso
16. 16what are bacteriorhodopsin used for

    1. Aa light driven proton pump used for photosynthesis in absence of chlorophyll
    2. Bused to produce energy in absence of mitochondria
    3. Ca light driven electron pump used for photosynthesis in absence of chlorophyll

Descripción

Test sobre Bacteria and Archaea, creado por Ryan Cornwell el 25/04/2019.

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Ryan Cornwell

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