Histology

1. 1Carbohydrates can be split into 2 functional groups, [blank_start]glycogen[blank_end] and [blank_start]mucins[blank_end]. Glycogen is a [blank_start]simple[blank_end] polysaccharide which functions as a glucose [blank_start]precursor[blank_end]. Mucins include [blank_start]glycoconjugates[blank_end], [blank_start]mucopolysaccharides[blank_end] and [blank_start]mucosubstances[blank_end]. They are often found as a [blank_start]mixture[blank_end].

   1. Aglycogen
   2. Bmucins
   3. Csimple
   4. Dprecursor
   5. Eglycoconjugates
   6. Fmucopolysaccharides
   7. Gmucosubstances
   8. Hmixture
2. 2Sometimes called the connective tissue mucins

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
3. 3All members are PAS positive

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
4. 4All members are PAS negative

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
5. 5Glycogen is the most important member of this group

   1. AGroup i: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
6. 6The term acid mucosubstance is used to include the acidic members

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
7. 7Includes cerebrosides and phosphatides

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup III: Glycoproteins
   4. DGroup IV: Glycolipids
8. 8Sometimes called the epithelial mucins

   1. AGroup I: Neutral Polysaccharides
   2. BGroup II: Acid Mucopolysaccharides
   3. CGroup II: Glycoproteins
   4. DGroup IV: Glycolipids
9. 9Schiff's reagent is formed by the reduction of basic [blank_start]fuchsin[blank_end], which is [blank_start]red[blank_end] to the colorless compound known as [blank_start]leucofuchsin[blank_end] or Schiff's reagent. The [blank_start]aldehyde[blank_end] groups in tissue [blank_start]oxidize[blank_end] the Schiff's reagent and impart a magenta or rose color to the dye.

   1. Afuchsin
   2. Bred
   3. Cleucofuchsin
   4. Daldehyde
   5. Eoxidize
10. 10What is the function of activated charcoal in the preparation of Schiff's reagent?

    1. AIt aids in reduction
    2. BIt removed impurities
    3. CIt prevents over-oxidation
    4. DIt prevents precipitation of dye molecules

11. 11How should good Schiff's reagent appear if formaldehyde is added to test it prior to staining?

    1. APurple-red
    2. BStraw color
    3. CColorless
    4. DBluish-red
12. 12What is the function of periodic acid in the PAS method?

    1. Areduces glycol fragments to aldehydes
    2. Breduces glycogen to simple sugars
    3. Coxidizes glycol groups to aldehydes
    4. Doxidizes glycogen to simple sugars
13. 13How should Schiff's reagent be stored?

    1. AIt must be stored frozen in a chemically clean container
    2. BIt must be made fresh daily so should never be stored
    3. CIt should be stored at room temperature in a dark container
    4. DIt should be refrigerated in a dark container
14. 14PAS-positive substances include which of the following?

    1. Aglycogen, fungal walls, thyroid colloid
    2. Bglycogen, fungal walls, mast cells
    3. Cglycogen, thyroid colloid, mast cells
    4. Dfungal walls, thyroid colloid, mast cells
15. 15Which of the following is true when performing the PAS method with diastase or alpha amylase digestion?

    1. Athe periodic acid step should be omitted
    2. Bany section of kidney would make a good control
    3. Cacid mucopolysaccharides will be reduced to glucose and maltose
    4. Dglycogen will be removed on the treated slide
16. 16What would the expected results be if the PAS reaction with alpha amylase is performed on a section of liver containing glycogen?

    1. Atreated slide - glycogen magenta; untreated slide - no glycogen staining
    2. Btreated slide - no glycogen staining; untreated slide - glycogen magenta
    3. Ctreated side - glycogen magenta; untreated slide - glycogen magenta
    4. Dtreated slide - glycogen colorless; untreated slide - glycogen colorless

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Test sobre Histology, creado por Natalie Collins el 19/05/2017.

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Natalie Collins

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