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HPLC and capillary electrophoresis: key concepts
Fichas del conjunto (7)
- 1advantages of HPLCrapid and precise analysis
high sensitivity detection
high resolution - 2types of stationary phase in HPLCnon permeable eg silica gel
permeable eg poymeric material
hydrophilic surface
hydrophobic surface - 3what is electroosmotic flow (EOF)the main driving force in capillary zone electrophoresis. capillary wall has a negative charge which develops a double layer of cations attracted to it. inner cation is stationary while the outer layer is free to move along the capillary
- 4what is reversed phase HPLCpolar mobile phase
hydrophobic stationary phase
used to analyse non polar drugs
seperation based on analytes partition coefficient between mobile phase and bonded stationary phase - 5what is ionic exchange used forto separate ionic analytes
- 6what are the requirements for mobile phasevery pure
low cost
UV transparency
low viscoisty
low toxicity
non flammable
de-gassed remove oxygen dissolved-no bubbles - 7amines
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- Fichas sobre Sin título, creado por Roisin Foster el 09/04/2017.
- Creado por
- Roisin Foster
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