Flashcards
automated enzyme
Cards in this deck (22)
- 1what form of CK is found in the brain?CK-BB
- 2what type of CK is found in the heart?CK-MB
- 3what type of CK is found in skeletal muscle?CK-MM
- 4what other enzymes raise in a myocardial infarction?lactate dehydrogenase
beta hydroxy butyrate dehydrogenase
Creatine phospokinase
aspartate aminotransferase - 5in enzymology, the enzyme is first reacted with something and then what is used to setect the enzyme?Spectrophetometry
Fluorimetry
electrodes (PH, Conductivity etc)
Calorimetry - 6after the enzymatic reaction, what conditions needs to be maintained to optimally detect the enzyme- or extract it?PH
ionic strength
temperature
free from inhibitors
optimal co-enzyme/co-factors
optimal substrate concentration - 7what would you do if the enzyme has more than one substrate?vary the concentration of one substrate, whilst maintaining a high concentration of another, so that the rate of reaction is dependent on the varied substrate
- 8why are coupled reactions sometimes used?when either the substrate or the product cannot be detected easily, therefore another product is needed
- 9what is an issue with coupled reactions?lag phase
- 10name one enzyme that requires a coupled reaction to be detected ?AST
- 11what coupled reaction does AST use?
- 12what are the major sources of enzymes?animals, plants and microbes
- 13name some animal sources of enzymes?Lipase
Ox Bile
Urokinase
Lysozyme- chicken albumen (eggs)
Adenosine deaminase
Pepsin
Dornase-a (human recombinant cells) - 14name some plant sources of enzymes?Papain
NattoKinase
amylase
Bromelain - 15Name some microbiological sources of enzymes?Beta Lactamase
Staphylokinase
Rhodanese
Streptokinase
L-asparaginase
Collagenase
Amylase - 16what is the preferred source of enzymes?Microbes
- 17why are microbes the preferred choice?- cheaper to produce
- reliable controlled source of enzyme
- reliable source for raw material for decomposition
- other sources contain harmful compounds, endogenous inhibitors and proteases - 18Sarcosidase is an orally used protein used to treat what?congenital sucrase-isomaltase deficiency
(CSID) - 19where is sarcosidase extracted from?Sacchromyces cerevisiae
- 20phenylase is an orally used protein for the treatment of what?PKU
- 21Dornase-alpha (pulmozyme) is an inhaled protein used for what?cystic fibrosis treatment
- 22—
Information
- Description
- 6 analytical methods Flashcards on automated enzyme, created by David Fox on 02/01/2018.
- Created by
- David Fox
- Views
- 4
- Rating
More on Diagnostic Enzymes and Biomarkers