Chapter 3 flashcards- Biochemical processes in cells

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Year 11 Biology (Chapter 3: Biochemical Processses in Cells) Flashcards on Chapter 3 flashcards- Biochemical processes in cells, created by Danielle J. Gills on 25/08/2014.
Danielle J. Gills
Flashcards by Danielle J. Gills, updated more than 1 year ago
Danielle J. Gills
Created by Danielle J. Gills over 11 years ago
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Question Answer
ATP Adenosine Triphosphate *ATP is the only form of energy directly used by the cell * when ATP is broken down to ADP and inorganic Phosphate, energy is released for use within the cell
Heterotroph *organisms that ingest or absorb organic matter of other living or dead organisms and their products *consumer
Autotroph *organisms which build organic compounds fro inorganic compounds *producer
Exergonic Reactions (Catabolic) *release energy * the energy of the reactants is greater than the energy of the products so energy is released
Endergonic Reaction *anabolic- needs energy *the energy of the products is greater than the energy of the reactants so energy is absorbed in the bonds of the products
Types Of Reactions Exergonic- those that release energy (catabolic) Endergonic- those that need energy (anabolic)
Enzyme Definition a protein molecule that speeds up a chemical reaction at temperatures normally found inside cells, by lowering the activation energy. They are biological catalysts.
Active Site Definition *The region on an enzyme surface that binds the substrate during the reaction. *tertiary folding of protein
Substrate Reactant in an enzyme catalysed reaction
Products Substance produced when the substrate has been changed by the enzyme
Enzyme Substrate Complex intermediate compound formed during the reaction as the enzyme and substrate are interacting at the active site.
Intracellular Enzymes Enzymes made inside the cell and remain there to speed up and control metabolism
Extracellular Enzymes enzymes made inside the cell but achieve their effect outside of the cell e.g digestive enzymes
Cofactors Organic molecules that are required by certain enzymes to carry out catalysis e.g vitamins, NAD,FAD
Coenzymes inorganic molecvules that assist enzymes in biochemical transformations e.g ions such as Mg+
The concept of the gene *metabolism is controlled by enzymes *ALL ENZYMES ARE PROTEINS *genes code for proteins *enzymes which are not functioning usually suggest the gene is faulty (inherited condition)
Enzyme-Substrate Specificity *Enzymes are specific and will only catalyse a certain reaction. for e.g maltase will only catalyse breakdown of the substrate maltose into glucose *active site matches the shape and chemical properties of the substrate
naming enzymes named by attaching the suffix -ase to the substrate of which it acts on e.g. Sucrose- Sucrase Exceptions: pepsin & trypsin
Lock and key model *this theory states that each enzyme has a particular active site with a configuration only the substrate can fit *active site is the 'lock' and the substrate is the 'key'. *The enzyme than catalyses the reaction, releasing the products leaving the enzymes unchanged *Active site is so specific it can only catalyse one reaction
Induced Fit Model *theory is that the active site is not a set structure for the substrate, but that as the substrate enters the active site, it induce it to change shape so that it fits the substrate more thoroughly *the active site alters so that several different but similar substrates can bind to it successfully
effect of temperature on enzyme activity *high temperature results in the chemical bonds of the tertiary structure breaking and the enzyme is denatured *therefore the active site alters *the substrate can no longer fit into the active site- substrate complex is not formed. *typical optimum temp 37 degrees
effect of pH on enzyme activity *changing the pH outside the limited range in which the enzyme normally works results in denaturation
effect of substrate concentration on enzyme activity *the more substrate there is, the greater the chance of a substrate molecule binding to an enzyme. *once all the enzymes are all taken up, the enzyme cannot work any faster and the only way to increase would be to increase enzyme concentration. * Between B and C the enzyme concentration is limiting
Enzyme Concentration *reaction rate increase with increasing enzyme concentration until the substrate becomes limiting
Enzyme Inhibition *Build up of end products slow down reaction rate and eventually reverse the reaction *enzyme poisons and inhibitors- certain enzymes are destroyed by particular poisons or are stopped by inhibitors
Types of inhibitors *competitive inhibitor- inhibitors mimic the substrate and block the active site *non competitive inhibitor- binds to another part of the enzyme causing active site to change shape
cycle of energy
Coenzymes
Equation for Respiration
Photosynthesis Equation
Photosynthesis *the process by which plants capture light energy and use it to covert the inorganic substances, water and carbon dioxide, to glucose. *oxygen is released as a by-product *light energy is trapped by chlorophyll and the process occurs in the chloroplast
Chlorophyll *green pigment molecule used to trap light in photosynthesis *chlorophyll absorbs most at the red and blue ends of the spectrum *these are the most effective wavelengths in photosynthesis
chloroplast structure
Structures in Chloroplasts
Stages in Photosynthesis Stage 1: light dependant reaction (photolysis) (water --> oxygen) Stage 2: light independent reaction (Calvin cycle) (carbon dioxide --> glucose)
Photosynthesis Overview
Light dependant reaction (photolysis) *occurs in thylakoid membranes-granum * light is trapped by chlorophyll *energy is used to split water into hydrogen ions (H+) and oxygen (released as a by product) *H+ picked up by carrier molecule (NADP+) and carried into the next stage *energy is used to make ATP, which is also used in the next stage
Photolysis equation
Light Independent Reaction ( Calvin cycle) *occurs in stroma * formation of glucose using H+ ions carried by NADPH *energy supplied by ATP from photolysis *Carbon dioxide used from atmosphere to make glucose *other carbohydrates can be produced from glucose
Calvin Cycle Equation
Summary reactions of photosynthesis
Cellular Respiration *process all living organisms use to obtain their energy * the controlled release of energy in the form of ATP from the substrate glucose in a series of chemical reactions *catabolic reaction *can be -AEROBIC (oxygen) -ANAEROBIC (no or low oxygen)
comparison of anaerobic and aerobic
Stages Of Aerobic Respiration Stage 1: Glycolysis (cytosol) (glucose --> 2 pyravte) Stage 2: Krebs Cycle (mitochondrion) (pyruvate +acetyl coenzyme A --> Carbon dioxide) Stage 3: Electron Transport Chain (mitochondrion) (H+ electrons + oxygen --> water)
overview of cellular respiration
Glycolysis *occurs in the cytosol *breakdown of one molecule of glucose into two pyruvate molecules (3 carbon compound) and a small net yield of ATP *hydrogen ions are collected by acceptor (NAD) which becomes NADH *pyruvic acid can now go into either aerobic or anaerobic respiration pathways
Glycolysis Equation
mitochondria *vary in length (1.5-10 micrometres long, 0.25-1 micrometres wide) *have a double membrane. *the inner one is highly folded to form cristae (increases SA) *contain DNA and ribosomes
mitochondrion structure
Krebs Cycle *occurs in matrix of mitochondria * if oxygen is present pyruvate molecules leave the cytosol and enter mitochondrion *pyruvate converted into acetyl coenzyme A *carbon dioxide is produced *acceptor molecules NADH and FADH2 (carrying electrons) move to the cristae for next stage
Krebs Cycle Equation
Electron Transport Chain (oxidative phosphorylation) *occurs in cristae of mitochondrion electrons are passed along CYTOCHROMES embedded in the membrane *oxygen is the final acceptor of electrons, together with H+ water is formed *electron transport produces large amounts of ATP
Electron Transport Chain Equation
Summary of Respiration
Rates of respiration and Photosynthesis *during the day rate of photosynthesis >respiration and glucose is converted into sucrose (used for energy or storage) *at night respiration > photosynthesis and the starch is converted back into glucose and used in cellular respiration
Photosynthesis vs. Respiration *process appear to be the opposite of each BUT... *living things respire ALL the time *photosynthesis only occurs during the day (no sunlight at night) * as light intensity increases, rate of photosynthesis increases unless a factor become limiting
Anaerobic Respiration *the chemical breakdown of glucose in the ABSENCE OF OXYGEN *occurs in cytoplasm *first stage is glycolysis Products: (mammal muscle and some bacteria)-lactic acid and water (plants)- ethanol and carbon dioxide
Anaerobic Respiration Equation
In event of Starvation..
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