16487429
Description
Flashcards by Courtney Miller, updated more than 1 year ago
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Created by Courtney Miller
over 6 years ago
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| Question | Answer |
| 7 Sterilization methods | 1. Autoclave 2. Radiation 3. Dry Heat 4. Boiling 5. Flashing 6. Gas 7.Cold disinfectant |
| Autoclaving: 3 modes of action | Heat, steam, pressure |
| What is the purpose of heat in the autoclave? | To denature microbial proteins |
| What is the purpose of steam in the autoclave? | Supplies the heat, promotes penetration of the heat |
| What is the purpose of pressure in the autoclave? | Adequately heats the steam |
| 3 factors affecting autoclaving | Temperature, Pressure, Exposure time |
| Minimums for Autoclaving (Temp, PSI, time) | 275° F - 15 PSI - 10 min 250° F - 15 PSI - 15 min |
| Advantages/Disadvantages: Autoclave | Adv: Economical, consistent, safe DIsadv: Dulls sharp instruments, need pressurized equipment, scorches fabric if too dry, damage instruments if not dried properly |
| Flashing: 1. Typically used for? 2. Temp - PSI - Time | 1. Individual instruments (may have been dropped during surgery) 2. 275° F - 32 psi - 4 minutes |
| How does boiling water sterilize? | Denaturing microbial proteins |
| Boiling Water: Time for partly sterilized Time for fully sterilized | Partly - 20 min Fully - 3+ hrs |
| Boiling Water: Advantages, disadvantages | Adv: Good for field work Disadv: Rarely used, autoclave is better |
| Dry heat: Mode of action Uses | Mode of action: oxidation denatures proteins Uses: Powders and non-aqueous solutions, animal care facilities |
| Dry heat: Advantages, disadvantages | Adv: Will not dull cutting edges Disadv: Hot air oven needed |
| Gas sterilization: What gas is used? Mode of action? | Gas: Ethylene Oxide Mode of action: Destroys metabolic pathways in pathogens |
| Gas sterilization: Advantages, disadvantages | Adv: Good for heat-sensitive materials, electrical & optical equipment, will not dull instruments Disadv: Expensive, flammable, dangerous, carcinogenic, OSHA regulations |
| Radiation: Mode of action Uses | Mode of action: damages cell enzymes and DNA Uses: Commercial preparation of pharmaceuticals, biologic products & disposable plastics |
| Cold "Sterilization": Mode of action Advantages, disadvantages | Mode of action: destroys metabolic pathways in microorganisms Advantages: Will not dull instruments, good for field instruments, not inactivated by organic material Disadvantages: Must be at correct concentrations, rusts, cause tissue damage |
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