Flashcards

Microtomy Troubleshoot

Vilmida Castro English 33 cards

Cards in this deck (33)

1Crooked Ribbons 1Cause: Horizontal edges of block not parallel
Remedy: Trim block until parallel. re position specimen holder
2Crooked Ribbons 2Cause: Blade blunt in one area
Remedy: Move blade or replace
3Crooked RibbonsCause: Tissue varies in consistency
Remedy: Rotate Block
4Holes in Section 1Cause: Block faced too aggressively
Remedy: Face block less aggressively
5Holes in Section 2Cause: excessive dehydration
Remedy: rehydrate
6A Ribbon Will Not Form 1Cause: Dull Knife
7Ribbon Will not Form 2Cause: Paraffin too hard
Remedy: Warm paraffin, re-embed in lower melting point paraffin
8A Ribbon Will Not Form 3Cause: too much knife tilt
Remedy: Adjust knife angle
9Ribbon Will Not Form 4Cause: Room temp too low
Remedy: Adjust room temp, warm knife and block
10Ribbon will not form 5Cause: sections are too thick
Remedy: cut thinner sections
11Sections Lift from Knife 1Cause: dull knife
Remedy: sharpen knife
12Sections lift from knife 2Cause: knife angle too little
Remedy: adjust knife tilt
13Sections lift from knife 3cause: warm room or paraffin too soft. block too warm
Remedy: chill block
14Washboarding or undulationsCause: knife angle is too great
remedy: decrease knife tilt
15Washboarding or undulations 2Cause: loose block or knife
remedy: tighten block or knife
16washboarding or undulationscause: hard tissue
remedy: use very sharp knife
17chatter or microscopic vibrationcause: over dehydration
remedy: rehydrate
18Chatter or microscopic vibration 2cause: too much knife tilt
remedy: adjust knife angle
19chatter or microscopic vibration 3cause: dull knife
remedy: sharpen knife or replace blade
20Alternate Thick and ThinCause: block or blade loose
remedy: tighten
21alternate thick & thin sectionscause: too little knife tilt
remedy: increase clearance angle
22alternate thick & thin sections 3cause: wax too soft
remedy: cool block or change to higher melting point wax
23compressed, wrinkled, jammed sectionscause: knife too dull or dirty
remedy: sharpen knife or replace blade, or clean blade or knife
24compressed, wrinkled, or jammed sectionscause: too little knife tilt
remedy: increase knife angle
25compressed sectionscause: block to warm
remedy: chill block
26compressed sections 2cause: cutting too quickly
remedy: slow down cutting speed
27lengthwise scrathescause: defect in knife edge or hard particle in the tissue or dirty knife
remedy: move or change the blade
28Scratches in ribbon
29Splits in the Ribbon
30Calcium in Tissue
31Sections fly and stick to itemscause: static electricity
remedy: add moisture to the air, grounding the microtome, static spray, dryer sheets
32sections disintegrate in water bathcause: poorly processed tissue or water bath too hot
remedy: reprocess or reduce temp of water bath
33sections too thick